Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28776 | 5' | -56.3 | NC_006146.1 | + | 23529 | 0.67 | 0.870778 |
Target: 5'- -aACUcGGGACUCCcGuGUCUGGCGg-- -3' miRNA: 3'- aaUGA-CCCUGAGGaC-CAGGCUGCacc -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 10096 | 0.68 | 0.848067 |
Target: 5'- gUUugUGaGGAC-CCcGGUCgaGGCGUGGg -3' miRNA: 3'- -AAugAC-CCUGaGGaCCAGg-CUGCACC- -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 71237 | 0.68 | 0.840106 |
Target: 5'- -gGCUGGucaACUCCUGGggcCUGGCGgGGg -3' miRNA: 3'- aaUGACCc--UGAGGACCa--GGCUGCaCC- -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 13503 | 0.68 | 0.828653 |
Target: 5'- -gGCUGGGAa-CCUGGgcuacuuuaugggCCGagGCGUGGa -3' miRNA: 3'- aaUGACCCUgaGGACCa------------GGC--UGCACC- -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 165811 | 0.68 | 0.823639 |
Target: 5'- -gGCUaggGGGGCgucCCUGG-CCG-CGUGGa -3' miRNA: 3'- aaUGA---CCCUGa--GGACCaGGCuGCACC- -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 33949 | 0.71 | 0.652914 |
Target: 5'- -cGCUGGGuC-CgCUGGUCCGgguggGCGUGGu -3' miRNA: 3'- aaUGACCCuGaG-GACCAGGC-----UGCACC- -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 33763 | 0.71 | 0.652914 |
Target: 5'- -cGCUGGGuC-CgCUGGUCCGgguggGCGUGGu -3' miRNA: 3'- aaUGACCCuGaG-GACCAGGC-----UGCACC- -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 46883 | 0.73 | 0.572315 |
Target: 5'- ----aGGGGCUCCUGGgugUCCGACGa-- -3' miRNA: 3'- aaugaCCCUGAGGACC---AGGCUGCacc -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 52863 | 0.73 | 0.562356 |
Target: 5'- -cGCccgGGGGCUCCaGGUCCGugGUc- -3' miRNA: 3'- aaUGa--CCCUGAGGaCCAGGCugCAcc -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 33322 | 0.73 | 0.552443 |
Target: 5'- --cCUGGGGCUCCggGGUCCGG-GUGc -3' miRNA: 3'- aauGACCCUGAGGa-CCAGGCUgCACc -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 121349 | 0.75 | 0.43906 |
Target: 5'- aUGCUGGG---CCUGG-CCGGCGUGGc -3' miRNA: 3'- aAUGACCCugaGGACCaGGCUGCACC- -5' |
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28776 | 5' | -56.3 | NC_006146.1 | + | 124215 | 1.08 | 0.003578 |
Target: 5'- cUUACUGGGACUCCUGGUCCGACGUGGa -3' miRNA: 3'- -AAUGACCCUGAGGACCAGGCUGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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