Results 1 - 20 of 120 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28780 | 3' | -62.5 | NC_006146.1 | + | 1154 | 0.66 | 0.593809 |
Target: 5'- aGGGUCC-CCGGgccgCCCCGGGgcucccccgcGCCGAu -3' miRNA: 3'- gUCCGGGaGGUCa---GGGGCCUa---------CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 2086 | 0.66 | 0.593809 |
Target: 5'- aGGGUCC-CCGGgccgCCCCGGGgcucccccgcGCCGAu -3' miRNA: 3'- gUCCGGGaGGUCa---GGGGCCUa---------CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 2759 | 0.72 | 0.270884 |
Target: 5'- -cGGCCCUCgCAGgcccaagCCCGGAUGUCAGg -3' miRNA: 3'- guCCGGGAG-GUCag-----GGGCCUACGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 3018 | 0.66 | 0.593809 |
Target: 5'- aGGGUCC-CCGGgccgCCCCGGGgcucccccgcGCCGAu -3' miRNA: 3'- gUCCGGGaGGUCa---GGGGCCUa---------CGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 3745 | 0.68 | 0.51083 |
Target: 5'- -uGGCCCUUCAG-CCCgGGGuggguccccuagaccUGCCAc -3' miRNA: 3'- guCCGGGAGGUCaGGGgCCU---------------ACGGUu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 10146 | 0.67 | 0.55475 |
Target: 5'- uCAGGCCCugucugaaaugaUCCAGUUuuaCCUGGAggaaguaaUGCCAc -3' miRNA: 3'- -GUCCGGG------------AGGUCAG---GGGCCU--------ACGGUu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 12360 | 0.74 | 0.219687 |
Target: 5'- uCAGGCCCUCCGcGUCCCCGuccuCCAGa -3' miRNA: 3'- -GUCCGGGAGGU-CAGGGGCcuacGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 13601 | 0.72 | 0.308029 |
Target: 5'- aGGGCCCUCCAGUCcagacccagagacgCCCaGGAgGCCc- -3' miRNA: 3'- gUCCGGGAGGUCAG--------------GGG-CCUaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 13841 | 0.68 | 0.479318 |
Target: 5'- uCAGGCCggCCGGagggaCCCCGGcgGCCc- -3' miRNA: 3'- -GUCCGGgaGGUCa----GGGGCCuaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 14057 | 0.7 | 0.384682 |
Target: 5'- cCAGGCCCUCC-GUCCUCccugGCCAc -3' miRNA: 3'- -GUCCGGGAGGuCAGGGGccuaCGGUu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 14881 | 0.67 | 0.574203 |
Target: 5'- cCAGGCCCUCCgAGgCCCCag--GCCu- -3' miRNA: 3'- -GUCCGGGAGG-UCaGGGGccuaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 15310 | 0.68 | 0.507077 |
Target: 5'- gGGGCCggaggCCAG-CCCCGGuGUGCCc- -3' miRNA: 3'- gUCCGGga---GGUCaGGGGCC-UACGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 15439 | 0.74 | 0.219687 |
Target: 5'- uCAGGCCCUCCGcGUCCCCGuccuCCAGa -3' miRNA: 3'- -GUCCGGGAGGU-CAGGGGCcuacGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 16679 | 0.72 | 0.308029 |
Target: 5'- aGGGCCCUCCAGUCcagacccagagacgCCCaGGAgGCCc- -3' miRNA: 3'- gUCCGGGAGGUCAG--------------GGG-CCUaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 16919 | 0.68 | 0.479318 |
Target: 5'- uCAGGCCggCCGGagggaCCCCGGcgGCCc- -3' miRNA: 3'- -GUCCGGgaGGUCa----GGGGCCuaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 17135 | 0.7 | 0.384682 |
Target: 5'- cCAGGCCCUCC-GUCCUCccugGCCAc -3' miRNA: 3'- -GUCCGGGAGGuCAGGGGccuaCGGUu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 18517 | 0.74 | 0.219687 |
Target: 5'- uCAGGCCCUCCGcGUCCCCGuccuCCAGa -3' miRNA: 3'- -GUCCGGGAGGU-CAGGGGCcuacGGUU- -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 19757 | 0.72 | 0.308029 |
Target: 5'- aGGGCCCUCCAGUCcagacccagagacgCCCaGGAgGCCc- -3' miRNA: 3'- gUCCGGGAGGUCAG--------------GGG-CCUaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 19996 | 0.68 | 0.479318 |
Target: 5'- uCAGGCCggCCGGagggaCCCCGGcgGCCc- -3' miRNA: 3'- -GUCCGGgaGGUCa----GGGGCCuaCGGuu -5' |
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28780 | 3' | -62.5 | NC_006146.1 | + | 20213 | 0.7 | 0.384682 |
Target: 5'- cCAGGCCCUCC-GUCCUCccugGCCAc -3' miRNA: 3'- -GUCCGGGAGGuCAGGGGccuaCGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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