Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28876 | 5' | -53.4 | NC_006146.1 | + | 57334 | 0.7 | 0.786037 |
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Target: 5'- cUCuCUGGGGGCGGGGC-CccGGGUg- -3' miRNA: 3'- aAG-GAUUCCCGCCUCGuGuaCCUAau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 45061 | 0.67 | 0.935917 |
|
Target: 5'- -cCCUAGGGGCGGcugaGGUGCcucGUGGGg-- -3' miRNA: 3'- aaGGAUUCCCGCC----UCGUG---UACCUaau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 41262 | 0.67 | 0.935917 |
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Target: 5'- aUUCCc--GGGCGGAGCACGcgcccgcGGAg-- -3' miRNA: 3'- -AAGGauuCCCGCCUCGUGUa------CCUaau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 34593 | 0.66 | 0.950027 |
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Target: 5'- -aCCUAGuGGGUGGAGC-C-UGGAg-- -3' miRNA: 3'- aaGGAUU-CCCGCCUCGuGuACCUaau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 33028 | 0.67 | 0.935407 |
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Target: 5'- -cCCUGccggcccGGGGCGGGGC-CcgGGAc-- -3' miRNA: 3'- aaGGAU-------UCCCGCCUCGuGuaCCUaau -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 31893 | 1.05 | 0.007778 |
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Target: 5'- uUUCCUAAGGGCGGAGCACAUGGAUUAg -3' miRNA: 3'- -AAGGAUUCCCGCCUCGUGUACCUAAU- -5' |
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| 28876 | 5' | -53.4 | NC_006146.1 | + | 9622 | 0.66 | 0.954228 |
|
Target: 5'- aUUUCUGGGGGUGuaaGGCACgcGUGGAUg- -3' miRNA: 3'- -AAGGAUUCCCGCc--UCGUG--UACCUAau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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