Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28896 | 3' | -54.5 | NC_006146.1 | + | 151387 | 0.68 | 0.869022 |
Target: 5'- aGUC-CCGcugcuGCAGGCGGGggaugGCgcggcgGCAGCUg -3' miRNA: 3'- -CAGuGGU-----UGUUCGUCCa----CGa-----CGUCGA- -5' |
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28896 | 3' | -54.5 | NC_006146.1 | + | 154668 | 0.68 | 0.861305 |
Target: 5'- -gCACCGugGuGGCGGGgggcGCUGCuGCUg -3' miRNA: 3'- caGUGGUugU-UCGUCCa---CGACGuCGA- -5' |
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28896 | 3' | -54.5 | NC_006146.1 | + | 159918 | 0.7 | 0.763736 |
Target: 5'- cGUCACCGGCAGcGCcgccugcuuuAGGUGCacUGUGGCa -3' miRNA: 3'- -CAGUGGUUGUU-CG----------UCCACG--ACGUCGa -5' |
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28896 | 3' | -54.5 | NC_006146.1 | + | 166654 | 0.66 | 0.91648 |
Target: 5'- -aCGCCAgaGCGAGCGGGaagggGgaGCGGUg -3' miRNA: 3'- caGUGGU--UGUUCGUCCa----CgaCGUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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