Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28896 | 3' | -54.5 | NC_006146.1 | + | 88158 | 0.68 | 0.845225 |
Target: 5'- uUCACgagguguagaUAACAauGGCA-GUGCUGCAGCUg -3' miRNA: 3'- cAGUG----------GUUGU--UCGUcCACGACGUCGA- -5' |
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28896 | 3' | -54.5 | NC_006146.1 | + | 10389 | 0.68 | 0.853371 |
Target: 5'- gGUCGCgGcuCAGGguGGUGCUGU-GCUa -3' miRNA: 3'- -CAGUGgUu-GUUCguCCACGACGuCGA- -5' |
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28896 | 3' | -54.5 | NC_006146.1 | + | 10870 | 0.68 | 0.861305 |
Target: 5'- aUCACCGugGAGCcGG-GCUuCAGCa -3' miRNA: 3'- cAGUGGUugUUCGuCCaCGAcGUCGa -5' |
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28896 | 3' | -54.5 | NC_006146.1 | + | 115446 | 0.66 | 0.94295 |
Target: 5'- --gGCCAuCGAGCGGGUggccacgguacuGCUGuCGGCg -3' miRNA: 3'- cagUGGUuGUUCGUCCA------------CGAC-GUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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