Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29024 | 5' | -52.6 | NC_006146.1 | + | 47655 | 0.66 | 0.976384 |
Target: 5'- -cGCGGCCcgGCCCAGcGCggcggCcaggGUCUCGu -3' miRNA: 3'- gaUGCUGGuaCGGGUC-UGa----G----UAGAGU- -5' |
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29024 | 5' | -52.6 | NC_006146.1 | + | 53164 | 0.66 | 0.978798 |
Target: 5'- aCUGCGGCgGUGgCCggaAGGCUCGgcCUCGg -3' miRNA: 3'- -GAUGCUGgUACgGG---UCUGAGUa-GAGU- -5' |
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29024 | 5' | -52.6 | NC_006146.1 | + | 95711 | 0.66 | 0.981024 |
Target: 5'- cCUACGuCCAgGgCCAGAC-CAUCUa- -3' miRNA: 3'- -GAUGCuGGUaCgGGUCUGaGUAGAgu -5' |
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29024 | 5' | -52.6 | NC_006146.1 | + | 116138 | 0.66 | 0.981024 |
Target: 5'- -gGCGGCCcUGCgCuGGCUgGUCUCc -3' miRNA: 3'- gaUGCUGGuACGgGuCUGAgUAGAGu -5' |
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29024 | 5' | -52.6 | NC_006146.1 | + | 75772 | 0.66 | 0.984946 |
Target: 5'- -gGCgGACCAaGCCCAGgggGCUCuacucgggcGUCUCGg -3' miRNA: 3'- gaUG-CUGGUaCGGGUC---UGAG---------UAGAGU- -5' |
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29024 | 5' | -52.6 | NC_006146.1 | + | 149841 | 0.66 | 0.984946 |
Target: 5'- -gGCGGCCAggGCCUcGGCgaagcgcagCGUCUCGc -3' miRNA: 3'- gaUGCUGGUa-CGGGuCUGa--------GUAGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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