Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29091 | 5' | -49.8 | NC_006146.1 | + | 93733 | 0.66 | 0.996811 |
Target: 5'- cGGAgGUGAAuucgauguUgUCCCGGAUCAGc -3' miRNA: 3'- cUCUgCGCUUuu------AgAGGGCCUAGUUu -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 26271 | 0.66 | 0.996811 |
Target: 5'- cGGGugGCGGAAuuagCCUGGGUgGAGg -3' miRNA: 3'- -CUCugCGCUUUuagaGGGCCUAgUUU- -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 71462 | 0.66 | 0.99774 |
Target: 5'- cGAGGaGCGAGGGccucaugCUCCUGGAUCu-- -3' miRNA: 3'- -CUCUgCGCUUUUa------GAGGGCCUAGuuu -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 153094 | 0.66 | 0.998112 |
Target: 5'- gGGGGCGCGAGuagcacaugCUCUCGGAgccCGGGa -3' miRNA: 3'- -CUCUGCGCUUuua------GAGGGCCUa--GUUU- -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 68191 | 0.66 | 0.998112 |
Target: 5'- aGGACGCGGGcgcagcggCUCCgGGAggUCAGAc -3' miRNA: 3'- cUCUGCGCUUuua-----GAGGgCCU--AGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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