Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29091 | 5' | -49.8 | NC_006146.1 | + | 128845 | 0.67 | 0.994843 |
Target: 5'- -uGuCGCGA--AUCUCCCuaGGGUCGAGc -3' miRNA: 3'- cuCuGCGCUuuUAGAGGG--CCUAGUUU- -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 130693 | 0.67 | 0.991991 |
Target: 5'- aGGGGCGCG-AGAUCUgggCGGAUCAu- -3' miRNA: 3'- -CUCUGCGCuUUUAGAgg-GCCUAGUuu -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 140142 | 0.67 | 0.994843 |
Target: 5'- uGGugGCGggGGUgC-CCCGGGUaCAGu -3' miRNA: 3'- cUCugCGCuuUUA-GaGGGCCUA-GUUu -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 144270 | 0.66 | 0.996592 |
Target: 5'- uGAGAUGCGggGAgaccucagaggcaCUCUCGGAUguGGg -3' miRNA: 3'- -CUCUGCGCuuUUa------------GAGGGCCUAguUU- -5' |
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29091 | 5' | -49.8 | NC_006146.1 | + | 153094 | 0.66 | 0.998112 |
Target: 5'- gGGGGCGCGAGuagcacaugCUCUCGGAgccCGGGa -3' miRNA: 3'- -CUCUGCGCUUuua------GAGGGCCUa--GUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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