miRNA: 3'- -CUCUGCGCuUUUAGAgg-GCCUAGUuu -5'
miRNA: 3'- -CUCUG-CGCuuUUAGag--GGCCuaGUUU- -5'
miRNA: 3'- -CUCUG----CGCUUUUAGAGGGCCUa-GUUu -5'
miRNA: 3'- cuCUGCgCUU-UUAGAGGGCCUAGUUU- -5'
miRNA: 3'- -CUCUgCGCUUUUa------GAGGGCCUAGuuu -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29091 | 5' | -49.8 | NC_006146.1 | + | 130693 | 0.67 | 0.991991 |
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Target: 5'- aGGGGCGCG-AGAUCUgggCGGAUCAu- -3' miRNA: 3'- -CUCUGCGCuUUUAGAgg-GCCUAGUuu -5' |
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| 29091 | 5' | -49.8 | NC_006146.1 | + | 127494 | 0.69 | 0.972905 |
|
Target: 5'- gGAGACgGCGggGGUCgcagCCGGggCAGAg -3' miRNA: 3'- -CUCUG-CGCuuUUAGag--GGCCuaGUUU- -5' |
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| 29091 | 5' | -49.8 | NC_006146.1 | + | 101955 | 0.73 | 0.866857 |
|
Target: 5'- uGAGACaacgGCGGAGAUCUCCUGGGccCAGg -3' miRNA: 3'- -CUCUG----CGCUUUUAGAGGGCCUa-GUUu -5' |
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| 29091 | 5' | -49.8 | NC_006146.1 | + | 50179 | 0.77 | 0.6759 |
|
Target: 5'- -cGAUGgGGGuGAUCUCCCGGAUCAGGu -3' miRNA: 3'- cuCUGCgCUU-UUAGAGGGCCUAGUUU- -5' |
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| 29091 | 5' | -49.8 | NC_006146.1 | + | 71462 | 0.66 | 0.99774 |
|
Target: 5'- cGAGGaGCGAGGGccucaugCUCCUGGAUCu-- -3' miRNA: 3'- -CUCUgCGCUUUUa------GAGGGCCUAGuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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