Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29147 | 3' | -53.6 | NC_006146.1 | + | 71539 | 0.66 | 0.976485 |
Target: 5'- cUCAACGCGcgGCCCGgGcGCCGa----- -3' miRNA: 3'- -AGUUGCGCa-UGGGCgC-UGGCauacag -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 114143 | 0.66 | 0.973668 |
Target: 5'- --uGCGCGUGCuCCGCGAucaggccCCGgacGUCc -3' miRNA: 3'- aguUGCGCAUG-GGCGCU-------GGCauaCAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 49942 | 0.66 | 0.971188 |
Target: 5'- gCAGCGCGcACUCGUGACgGUucuUGg- -3' miRNA: 3'- aGUUGCGCaUGGGCGCUGgCAu--ACag -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 166779 | 0.66 | 0.968239 |
Target: 5'- gUCAcGCGCGUAgUCGCuGGCCGcc-GUCa -3' miRNA: 3'- -AGU-UGCGCAUgGGCG-CUGGCauaCAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 106639 | 0.66 | 0.968239 |
Target: 5'- gCAGCGCcUG-CUGCGGCCGgcugGUCa -3' miRNA: 3'- aGUUGCGcAUgGGCGCUGGCaua-CAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 85348 | 0.67 | 0.958124 |
Target: 5'- -----aCGUGCCCcCGACCGUGUGa- -3' miRNA: 3'- aguugcGCAUGGGcGCUGGCAUACag -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 102776 | 0.67 | 0.95431 |
Target: 5'- gCGugGCGUGgcgccgcuCCCGCGgACCGgcUGg- -3' miRNA: 3'- aGUugCGCAU--------GGGCGC-UGGCauACag -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 113453 | 0.68 | 0.926539 |
Target: 5'- -aAACGUG-AgCCGCGGCCcggcuuccucGUAUGUCa -3' miRNA: 3'- agUUGCGCaUgGGCGCUGG----------CAUACAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 111740 | 0.69 | 0.921078 |
Target: 5'- cCGACGaCGUGCUgGUGGCCcu-UGUCa -3' miRNA: 3'- aGUUGC-GCAUGGgCGCUGGcauACAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 49038 | 0.69 | 0.915377 |
Target: 5'- -gGGCGCG-GCCCGaGACCGUcgcGUCg -3' miRNA: 3'- agUUGCGCaUGGGCgCUGGCAua-CAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 166830 | 0.69 | 0.909438 |
Target: 5'- gUCGACGCG-GCCCGgGuCCGcGUGg- -3' miRNA: 3'- -AGUUGCGCaUGGGCgCuGGCaUACag -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 167425 | 0.69 | 0.903262 |
Target: 5'- cCAGCGCGggguCCCGgGGCgGgggGUCg -3' miRNA: 3'- aGUUGCGCau--GGGCgCUGgCauaCAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 170220 | 0.69 | 0.903262 |
Target: 5'- cCAGCGCGggguCCCGgGGCgGgggGUCg -3' miRNA: 3'- aGUUGCGCau--GGGCgCUGgCauaCAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 169512 | 0.69 | 0.903262 |
Target: 5'- cCGGCGCGUGCCgGgGGacCCGggggcGUGUCc -3' miRNA: 3'- aGUUGCGCAUGGgCgCU--GGCa----UACAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 170443 | 0.69 | 0.903262 |
Target: 5'- cCGGCGCGUGCCgGgGGacCCGggggcGUGUCc -3' miRNA: 3'- aGUUGCGCAUGGgCgCU--GGCa----UACAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 168580 | 0.69 | 0.903262 |
Target: 5'- cCGGCGCGUGCCgGgGGacCCGggggcGUGUCc -3' miRNA: 3'- aGUUGCGCAUGGgCgCU--GGCa----UACAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 168357 | 0.69 | 0.903262 |
Target: 5'- cCAGCGCGggguCCCGgGGCgGgggGUCg -3' miRNA: 3'- aGUUGCGCau--GGGCgCUGgCauaCAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 169289 | 0.69 | 0.903262 |
Target: 5'- cCAGCGCGggguCCCGgGGCgGgggGUCg -3' miRNA: 3'- aGUUGCGCau--GGGCgCUGgCauaCAG- -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 98308 | 0.69 | 0.896853 |
Target: 5'- -gAGgGCGUGCuCCGUGGCCGUcUGg- -3' miRNA: 3'- agUUgCGCAUG-GGCGCUGGCAuACag -5' |
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29147 | 3' | -53.6 | NC_006146.1 | + | 45908 | 0.7 | 0.883345 |
Target: 5'- uUCAGCGCccgGCCgGUGACCGUGccGUa -3' miRNA: 3'- -AGUUGCGca-UGGgCGCUGGCAUa-CAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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