Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29163 | 3' | -58.9 | NC_006150.1 | + | 4303 | 0.66 | 0.857391 |
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Target: 5'- gGUGAGCGAugAuaaaccCGCCAcacuguGGCUGUCGa -3' miRNA: 3'- gCGCUCGUUugUc-----GCGGU------CCGACGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 94229 | 0.66 | 0.857391 |
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Target: 5'- uGCGAGU--ACuGgGCgAGGgaGCCGg -3' miRNA: 3'- gCGCUCGuuUGuCgCGgUCCgaCGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 197280 | 0.66 | 0.864748 |
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Target: 5'- gCGCGAu--GAUAGCcguauacacaGCCAGGCUGCg- -3' miRNA: 3'- -GCGCUcguUUGUCG----------CGGUCCGACGgc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 182610 | 0.66 | 0.864748 |
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Target: 5'- aGCGAGCGcAGCGGgugguCGCaCAGGagggagGCCGg -3' miRNA: 3'- gCGCUCGU-UUGUC-----GCG-GUCCga----CGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 92742 | 0.66 | 0.864748 |
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Target: 5'- aGCGGGC--ACGGCGCUu--CUGCCu -3' miRNA: 3'- gCGCUCGuuUGUCGCGGuccGACGGc -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 92447 | 0.66 | 0.864748 |
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Target: 5'- gGCGAGgAGGCGGUGaCCGuGUcGCCGg -3' miRNA: 3'- gCGCUCgUUUGUCGC-GGUcCGaCGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 65169 | 0.66 | 0.87191 |
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Target: 5'- uGCaaacGGCcccCAGCGCCAcGGCcGCCGa -3' miRNA: 3'- gCGc---UCGuuuGUCGCGGU-CCGaCGGC- -5' |
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| 29163 | 3' | -58.9 | NC_006150.1 | + | 147854 | 0.66 | 0.87191 |
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Target: 5'- aCGCGcGUAGGCAGCuuGagAGaGCUGCCGc -3' miRNA: 3'- -GCGCuCGUUUGUCG--CggUC-CGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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