Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29177 | 3' | -57.8 | NC_006150.1 | + | 71038 | 0.66 | 0.881909 |
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Target: 5'- -cCGUUGGCCGCCGCCuuuuUGGcACaGAGu -3' miRNA: 3'- cuGUAGCCGGCGGUGGu---GCC-UG-CUUu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 102307 | 0.66 | 0.888616 |
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Target: 5'- uGCggCGGuuGCCGCgGCGG-CGAc- -3' miRNA: 3'- cUGuaGCCggCGGUGgUGCCuGCUuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 140726 | 0.66 | 0.89511 |
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Target: 5'- uGACcuuGUCGacgauCCGCUcgaucuuaGCCGCGGACGAGAg -3' miRNA: 3'- -CUG---UAGCc----GGCGG--------UGGUGCCUGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 117058 | 0.66 | 0.89511 |
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Target: 5'- cGGCGUCcauGGCCGCCAUgcggggCACGucCGAAAc -3' miRNA: 3'- -CUGUAG---CCGGCGGUG------GUGCcuGCUUU- -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 130668 | 0.66 | 0.901388 |
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Target: 5'- aACGUCugaggGGCgcagGCUGCCACGGGCGAc- -3' miRNA: 3'- cUGUAG-----CCGg---CGGUGGUGCCUGCUuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 130019 | 0.66 | 0.901388 |
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Target: 5'- uGACGUCGGUCaCgGCCGCgGGACa--- -3' miRNA: 3'- -CUGUAGCCGGcGgUGGUG-CCUGcuuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 48308 | 0.66 | 0.901388 |
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Target: 5'- cGACAUCGGCCuCCGUUuuGGGCGGu- -3' miRNA: 3'- -CUGUAGCCGGcGGUGGugCCUGCUuu -5' |
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| 29177 | 3' | -57.8 | NC_006150.1 | + | 110729 | 0.66 | 0.907446 |
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Target: 5'- gGACAUCGGuUCGU--CCGCGGGCGu-- -3' miRNA: 3'- -CUGUAGCC-GGCGguGGUGCCUGCuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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