Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29371 | 5' | -53.8 | NC_006151.1 | + | 53024 | 0.66 | 0.939946 |
Target: 5'- -cCAGCc-CugCAGGUUCGCCgGGUu -3' miRNA: 3'- acGUCGauGugGUUCAAGCGGaCCAu -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 50227 | 0.66 | 0.928013 |
Target: 5'- gUGCGGCUGCACCGGGacaacguguuugcggCGCCa---- -3' miRNA: 3'- -ACGUCGAUGUGGUUCaa-------------GCGGaccau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 48938 | 0.68 | 0.843299 |
Target: 5'- aGguGCUGC-CCGcgcgcgaggacguGUUCGCCUGGa- -3' miRNA: 3'- aCguCGAUGuGGUu------------CAAGCGGACCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 24738 | 0.66 | 0.934926 |
Target: 5'- gUGCGGCaGgACCAGGcacgUCGCCgGGc- -3' miRNA: 3'- -ACGUCGaUgUGGUUCa---AGCGGaCCau -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 3276 | 0.66 | 0.92411 |
Target: 5'- cGCGGCgaugUGCGCCAGGgcgGCCgGGUc -3' miRNA: 3'- aCGUCG----AUGUGGUUCaagCGGaCCAu -5' |
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29371 | 5' | -53.8 | NC_006151.1 | + | 2453 | 0.68 | 0.870647 |
Target: 5'- cGCAGCgggGCGCCGagcccccagcGGUUgGCCgcgcGGUGc -3' miRNA: 3'- aCGUCGa--UGUGGU----------UCAAgCGGa---CCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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