Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29373 | 3' | -54.5 | NC_006151.1 | + | 115428 | 0.66 | 0.940326 |
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Target: 5'- --uGGggGGGCggaagaggUCgCGCGUGaUGCGCGc -3' miRNA: 3'- gguUCuaCCCGa-------AG-GCGCAC-ACGUGC- -5' |
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| 29373 | 3' | -54.5 | NC_006151.1 | + | 117117 | 0.67 | 0.925079 |
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Target: 5'- aCCAGGccGGcGCacaccgUCuCGCGgagGUGCGCGa -3' miRNA: 3'- -GGUUCuaCC-CGa-----AG-GCGCa--CACGUGC- -5' |
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| 29373 | 3' | -54.5 | NC_006151.1 | + | 117584 | 0.67 | 0.925079 |
|
Target: 5'- -gAGGAUGGGC---CGCGUG-GCGCc -3' miRNA: 3'- ggUUCUACCCGaagGCGCACaCGUGc -5' |
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| 29373 | 3' | -54.5 | NC_006151.1 | + | 134236 | 0.71 | 0.762522 |
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Target: 5'- aCGGGAUcguggacgcGGGCUUUCGCGgcgagGUGCAgGc -3' miRNA: 3'- gGUUCUA---------CCCGAAGGCGCa----CACGUgC- -5' |
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| 29373 | 3' | -54.5 | NC_006151.1 | + | 137695 | 1.1 | 0.003447 |
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Target: 5'- gCCAAGAUGGGCUUCCGCGUGUGCACGc -3' miRNA: 3'- -GGUUCUACCCGAAGGCGCACACGUGC- -5' |
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| 29373 | 3' | -54.5 | NC_006151.1 | + | 141620 | 0.72 | 0.68294 |
|
Target: 5'- gCCGGGAUcGGGCgggucgcugCCGCGgcgGCGCGg -3' miRNA: 3'- -GGUUCUA-CCCGaa-------GGCGCacaCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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