Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29373 | 3' | -54.5 | NC_006151.1 | + | 3255 | 0.66 | 0.935486 |
Target: 5'- gCgGAGcgGGGCg-CCGCG-GcGCGCGg -3' miRNA: 3'- -GgUUCuaCCCGaaGGCGCaCaCGUGC- -5' |
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29373 | 3' | -54.5 | NC_006151.1 | + | 115428 | 0.66 | 0.940326 |
Target: 5'- --uGGggGGGCggaagaggUCgCGCGUGaUGCGCGc -3' miRNA: 3'- gguUCuaCCCGa-------AG-GCGCAC-ACGUGC- -5' |
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29373 | 3' | -54.5 | NC_006151.1 | + | 113680 | 0.66 | 0.940326 |
Target: 5'- gCCGAGAaccGCUUCgaggggCGCGUGUGCGgGg -3' miRNA: 3'- -GGUUCUaccCGAAG------GCGCACACGUgC- -5' |
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29373 | 3' | -54.5 | NC_006151.1 | + | 77374 | 0.66 | 0.944475 |
Target: 5'- gCCGacGGcgGGGa--CCGCGcguacuuUGUGCACGg -3' miRNA: 3'- -GGU--UCuaCCCgaaGGCGC-------ACACGUGC- -5' |
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29373 | 3' | -54.5 | NC_006151.1 | + | 111304 | 0.66 | 0.944925 |
Target: 5'- cCCAcGAacgcGGCggCCGCGUGcccggGCACGg -3' miRNA: 3'- -GGUuCUac--CCGaaGGCGCACa----CGUGC- -5' |
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29373 | 3' | -54.5 | NC_006151.1 | + | 75854 | 0.66 | 0.949285 |
Target: 5'- cCCGucGAcgGGGCgccccugcgugUCCGCGUGggucaUGCGCGc -3' miRNA: 3'- -GGUu-CUa-CCCGa----------AGGCGCAC-----ACGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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