Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29376 | 3' | -53.4 | NC_006151.1 | + | 127532 | 0.66 | 0.96522 |
Target: 5'- cGCGUCaGCggGGCCCA----GGGGGCg -3' miRNA: 3'- -UGCAGaUGgaCCGGGUgaaaCUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 91174 | 0.66 | 0.961773 |
Target: 5'- cACGUCgg---GcGCCCGCgagGGGAGCa -3' miRNA: 3'- -UGCAGauggaC-CGGGUGaaaCUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 11524 | 0.66 | 0.961773 |
Target: 5'- cGCGgcaCU-CCgGGCCCGCg-UGAGAGg -3' miRNA: 3'- -UGCa--GAuGGaCCGGGUGaaACUCUCg -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 97707 | 0.66 | 0.958096 |
Target: 5'- uGCGUC-ACgUGGCCCA-----GGAGCc -3' miRNA: 3'- -UGCAGaUGgACCGGGUgaaacUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 2734 | 0.66 | 0.954183 |
Target: 5'- gGCGaagcagGCCgGGCCCACgaUG-GAGCu -3' miRNA: 3'- -UGCaga---UGGaCCGGGUGaaACuCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 84351 | 0.67 | 0.945635 |
Target: 5'- uACGUC-GCC-GGCCCGCggcggUGAucGCg -3' miRNA: 3'- -UGCAGaUGGaCCGGGUGaa---ACUcuCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 713 | 0.67 | 0.945635 |
Target: 5'- cCGUCgcACCgggGGUCCGCgggcGGGGGCu -3' miRNA: 3'- uGCAGa-UGGa--CCGGGUGaaa-CUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 111292 | 0.67 | 0.945635 |
Target: 5'- uACG-CgGCCUGGCCCACgaacgcGGCg -3' miRNA: 3'- -UGCaGaUGGACCGGGUGaaacucUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 70910 | 0.67 | 0.945635 |
Target: 5'- uGCG-CUGCCUGGCgCGCg-UGcuGGCg -3' miRNA: 3'- -UGCaGAUGGACCGgGUGaaACucUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 22990 | 0.67 | 0.936102 |
Target: 5'- -gGUCgugAUCUcGGCCgCGCgggUGGGGGCg -3' miRNA: 3'- ugCAGa--UGGA-CCGG-GUGaa-ACUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 111463 | 0.68 | 0.915225 |
Target: 5'- uGCGUCUGCgaggcgucgucggGGCCCAUgaagcggaacGAGAGCu -3' miRNA: 3'- -UGCAGAUGga-----------CCGGGUGaaa-------CUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 132285 | 0.68 | 0.914626 |
Target: 5'- cACGUCcugggccaccACCUGGUCCGCgugcaggcgccacGAGAGCu -3' miRNA: 3'- -UGCAGa---------UGGACCGGGUGaaa----------CUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 119111 | 0.68 | 0.914026 |
Target: 5'- uACGUgaGCCgcgcGGCCgGCcucgUGGGGGCg -3' miRNA: 3'- -UGCAgaUGGa---CCGGgUGaa--ACUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 23140 | 0.68 | 0.901486 |
Target: 5'- cCGUC-GCCgGGCCCuCggcGGGAGCg -3' miRNA: 3'- uGCAGaUGGaCCGGGuGaaaCUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 6918 | 0.68 | 0.901486 |
Target: 5'- cGCGUCUACUUugcauguccGGCCCc----GAGGGCg -3' miRNA: 3'- -UGCAGAUGGA---------CCGGGugaaaCUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 106992 | 0.68 | 0.894848 |
Target: 5'- cGCGU--GCCUGGCgCCGCagcuGGAGCg -3' miRNA: 3'- -UGCAgaUGGACCG-GGUGaaacUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 9470 | 0.68 | 0.894848 |
Target: 5'- cGCGUCcGCUUcGGCCCcccgcggccgcgGCguggGAGAGCg -3' miRNA: 3'- -UGCAGaUGGA-CCGGG------------UGaaa-CUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 35570 | 0.68 | 0.887968 |
Target: 5'- gGCGaaaaUCgGCCauUGGUCCGCUUaccUGGGGGCg -3' miRNA: 3'- -UGC----AGaUGG--ACCGGGUGAA---ACUCUCG- -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 70022 | 0.69 | 0.880851 |
Target: 5'- cCGUCUGCacgUGGCCCGCgaacacgGAGAc- -3' miRNA: 3'- uGCAGAUGg--ACCGGGUGaaa----CUCUcg -5' |
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29376 | 3' | -53.4 | NC_006151.1 | + | 13989 | 0.7 | 0.807073 |
Target: 5'- cGCGUCc-CCggGGCCCACgugUGAG-GCc -3' miRNA: 3'- -UGCAGauGGa-CCGGGUGaa-ACUCuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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