Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29376 | 3' | -53.4 | NC_006151.1 | + | 124673 | 0.71 | 0.788631 |
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Target: 5'- uACGgcCUGCgCgagggGGCCCACg--GGGAGCa -3' miRNA: 3'- -UGCa-GAUG-Ga----CCGGGUGaaaCUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 105147 | 0.71 | 0.779182 |
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Target: 5'- cGCGUCgacgGCCgcgGGCCCAUg--GAcGAGUu -3' miRNA: 3'- -UGCAGa---UGGa--CCGGGUGaaaCU-CUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 121958 | 0.71 | 0.769595 |
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Target: 5'- gGCGaCgacGCCgaGGCCUACUUUGAGcGCa -3' miRNA: 3'- -UGCaGa--UGGa-CCGGGUGAAACUCuCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 110071 | 0.71 | 0.759879 |
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Target: 5'- gACGUCUcUCUGGCCCA-----GGAGCu -3' miRNA: 3'- -UGCAGAuGGACCGGGUgaaacUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 130916 | 0.74 | 0.615833 |
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Target: 5'- uCGUCggugGCCgGGCCCGCgggggcGGGGGCg -3' miRNA: 3'- uGCAGa---UGGaCCGGGUGaaa---CUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 64941 | 0.74 | 0.605351 |
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Target: 5'- cGCGUCagcGCCUGGCCgCGCUgcGGGcGCa -3' miRNA: 3'- -UGCAGa--UGGACCGG-GUGAaaCUCuCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 86122 | 0.74 | 0.602211 |
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Target: 5'- cCGgCUACCUGGCCCugUacgauagggacggcgGGGAGCu -3' miRNA: 3'- uGCaGAUGGACCGGGugAaa-------------CUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 136559 | 1.11 | 0.003169 |
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Target: 5'- uACGUCUACCUGGCCCACUUUGAGAGCu -3' miRNA: 3'- -UGCAGAUGGACCGGGUGAAACUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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