Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29376 | 3' | -53.4 | NC_006151.1 | + | 70910 | 0.67 | 0.945635 |
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Target: 5'- uGCG-CUGCCUGGCgCGCg-UGcuGGCg -3' miRNA: 3'- -UGCaGAUGGACCGgGUGaaACucUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 111292 | 0.67 | 0.945635 |
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Target: 5'- uACG-CgGCCUGGCCCACgaacgcGGCg -3' miRNA: 3'- -UGCaGaUGGACCGGGUGaaacucUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 713 | 0.67 | 0.945635 |
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Target: 5'- cCGUCgcACCgggGGUCCGCgggcGGGGGCu -3' miRNA: 3'- uGCAGa-UGGa--CCGGGUGaaa-CUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 84351 | 0.67 | 0.945635 |
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Target: 5'- uACGUC-GCC-GGCCCGCggcggUGAucGCg -3' miRNA: 3'- -UGCAGaUGGaCCGGGUGaa---ACUcuCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 2734 | 0.66 | 0.954183 |
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Target: 5'- gGCGaagcagGCCgGGCCCACgaUG-GAGCu -3' miRNA: 3'- -UGCaga---UGGaCCGGGUGaaACuCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 97707 | 0.66 | 0.958096 |
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Target: 5'- uGCGUC-ACgUGGCCCA-----GGAGCc -3' miRNA: 3'- -UGCAGaUGgACCGGGUgaaacUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 11524 | 0.66 | 0.961773 |
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Target: 5'- cGCGgcaCU-CCgGGCCCGCg-UGAGAGg -3' miRNA: 3'- -UGCa--GAuGGaCCGGGUGaaACUCUCg -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 91174 | 0.66 | 0.961773 |
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Target: 5'- cACGUCgg---GcGCCCGCgagGGGAGCa -3' miRNA: 3'- -UGCAGauggaC-CGGGUGaaaCUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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