Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29376 | 3' | -53.4 | NC_006151.1 | + | 111463 | 0.68 | 0.915225 |
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Target: 5'- uGCGUCUGCgaggcgucgucggGGCCCAUgaagcggaacGAGAGCu -3' miRNA: 3'- -UGCAGAUGga-----------CCGGGUGaaa-------CUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 119111 | 0.68 | 0.914026 |
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Target: 5'- uACGUgaGCCgcgcGGCCgGCcucgUGGGGGCg -3' miRNA: 3'- -UGCAgaUGGa---CCGGgUGaa--ACUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 121958 | 0.71 | 0.769595 |
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Target: 5'- gGCGaCgacGCCgaGGCCUACUUUGAGcGCa -3' miRNA: 3'- -UGCaGa--UGGa-CCGGGUGAAACUCuCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 124673 | 0.71 | 0.788631 |
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Target: 5'- uACGgcCUGCgCgagggGGCCCACg--GGGAGCa -3' miRNA: 3'- -UGCa-GAUG-Ga----CCGGGUGaaaCUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 127532 | 0.66 | 0.96522 |
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Target: 5'- cGCGUCaGCggGGCCCA----GGGGGCg -3' miRNA: 3'- -UGCAGaUGgaCCGGGUgaaaCUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 130916 | 0.74 | 0.615833 |
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Target: 5'- uCGUCggugGCCgGGCCCGCgggggcGGGGGCg -3' miRNA: 3'- uGCAGa---UGGaCCGGGUGaaa---CUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 132285 | 0.68 | 0.914626 |
|
Target: 5'- cACGUCcugggccaccACCUGGUCCGCgugcaggcgccacGAGAGCu -3' miRNA: 3'- -UGCAGa---------UGGACCGGGUGaaa----------CUCUCG- -5' |
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| 29376 | 3' | -53.4 | NC_006151.1 | + | 136559 | 1.11 | 0.003169 |
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Target: 5'- uACGUCUACCUGGCCCACUUUGAGAGCu -3' miRNA: 3'- -UGCAGAUGGACCGGGUGAAACUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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