miRNA: 3'- -CCUGa--UGUa-GAGGgUgCUGGCCCCGu -5'
miRNA: 3'- -CCUGauguaGAGGGuGCUgGCCCCGu -5'
miRNA: 3'- cCUGa-UGUagaGGG----UGCUGGCCCCGU- -5'
miRNA: 3'- -CCUGa---UG------UAGaGGGUGCUGGCCCcGU- -5'
miRNA: 3'- -CCUGa--UGUa---GAGGG-----UGCUGGCCCCGU- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29377 | 5' | -57.2 | NC_006151.1 | + | 5013 | 0.68 | 0.78252 |
|
Target: 5'- gGGGCcggGCGggCUCCgG-GGCCGGGGCc -3' miRNA: 3'- -CCUGa--UGUa-GAGGgUgCUGGCCCCGu -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 4909 | 0.66 | 0.842657 |
|
Target: 5'- cGGGCcagggCUCCC-CGAgCGGGGUg -3' miRNA: 3'- -CCUGauguaGAGGGuGCUgGCCCCGu -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 4200 | 0.68 | 0.754606 |
|
Target: 5'- aGGCagGCGgg-CCCgaggGCGGCCGGGGCGc -3' miRNA: 3'- cCUGa-UGUagaGGG----UGCUGGCCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 2843 | 0.68 | 0.754606 |
|
Target: 5'- cGGACguagACgugguaGUCcCCCACGGCCGGGcCGu -3' miRNA: 3'- -CCUGa---UG------UAGaGGGUGCUGGCCCcGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 637 | 0.75 | 0.398375 |
|
Target: 5'- cGGACgugACGccggCUUCCggggcGCGGCCGGGGCGg -3' miRNA: 3'- -CCUGa--UGUa---GAGGG-----UGCUGGCCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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