Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29377 | 5' | -57.2 | NC_006151.1 | + | 21367 | 0.67 | 0.834567 |
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Target: 5'- cGGGCUGCGgccaCCCgcugggcacgACGGCCggcGGGGCGa -3' miRNA: 3'- -CCUGAUGUaga-GGG----------UGCUGG---CCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 81576 | 0.67 | 0.834567 |
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Target: 5'- cGGAgCaGgGUCUCgCGCGcgucGCCGGGGCc -3' miRNA: 3'- -CCU-GaUgUAGAGgGUGC----UGGCCCCGu -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 45570 | 0.66 | 0.842657 |
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Target: 5'- cGGGC--CAUCgUCCCgggACGGCCcgagGGGGCGg -3' miRNA: 3'- -CCUGauGUAG-AGGG---UGCUGG----CCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 4909 | 0.66 | 0.842657 |
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Target: 5'- cGGGCcagggCUCCC-CGAgCGGGGUg -3' miRNA: 3'- -CCUGauguaGAGGGuGCUgGCCCCGu -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 132560 | 0.66 | 0.880182 |
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Target: 5'- cGGGCaGCuccUCcUCCACGGCCGGGacucGCGg -3' miRNA: 3'- -CCUGaUGu--AGaGGGUGCUGGCCC----CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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