Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29377 | 5' | -57.2 | NC_006151.1 | + | 135602 | 0.71 | 0.615627 |
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Target: 5'- cGGCU-CAUaagccCUCCCACGGCCGcGGCGc -3' miRNA: 3'- cCUGAuGUA-----GAGGGUGCUGGCcCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 136500 | 1.1 | 0.001864 |
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Target: 5'- gGGACUACAUCUCCCACGACCGGGGCAg -3' miRNA: 3'- -CCUGAUGUAGAGGGUGCUGGCCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 136883 | 0.66 | 0.842657 |
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Target: 5'- aGGAgcGCcUCgcgcCCCACGGCUGGGuGCGc -3' miRNA: 3'- -CCUgaUGuAGa---GGGUGCUGGCCC-CGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 141360 | 0.68 | 0.764028 |
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Target: 5'- aGGA--GCGUCUCCgagUAgGACgGGGGCGg -3' miRNA: 3'- -CCUgaUGUAGAGG---GUgCUGgCCCCGU- -5' |
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| 29377 | 5' | -57.2 | NC_006151.1 | + | 141666 | 0.67 | 0.834567 |
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Target: 5'- cGGGCUcguuguggagGCAUgucugcCUCCCACGgcgGCUGGcGGCGg -3' miRNA: 3'- -CCUGA----------UGUA------GAGGGUGC---UGGCC-CCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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