Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29380 | 5' | -49.7 | NC_006151.1 | + | 98947 | 0.66 | 0.995071 |
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Target: 5'- cGAGGcgGCGCgggccgUGgacGAGGCG-GCg -3' miRNA: 3'- uCUUCuaCGCGa-----ACaacUUCCGCaCG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 44736 | 0.66 | 0.995071 |
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Target: 5'- aAGAAGccgagcAUGCGCgcgggUGUUGu--GUGUGCg -3' miRNA: 3'- -UCUUC------UACGCGa----ACAACuucCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 42988 | 0.66 | 0.995071 |
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Target: 5'- gAGAagAGAUGCG---GggGAGGGCGgcggGCg -3' miRNA: 3'- -UCU--UCUACGCgaaCaaCUUCCGCa---CG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 15032 | 0.66 | 0.995516 |
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Target: 5'- gAGGAGGUGCGCguccac--GGCGcGCg -3' miRNA: 3'- -UCUUCUACGCGaacaacuuCCGCaCG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 52960 | 0.66 | 0.995794 |
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Target: 5'- -uGAGGUGCGCg---UGcAGGCG-GCu -3' miRNA: 3'- ucUUCUACGCGaacaACuUCCGCaCG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 20876 | 0.66 | 0.996428 |
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Target: 5'- uAGcAGgcGCGCUUGggGucGAGGCGcaGCa -3' miRNA: 3'- -UCuUCuaCGCGAACaaC--UUCCGCa-CG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 27296 | 0.66 | 0.996428 |
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Target: 5'- ------cGCGCUUG--GggGGCGUGg -3' miRNA: 3'- ucuucuaCGCGAACaaCuuCCGCACg -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 8564 | 0.66 | 0.996428 |
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Target: 5'- gAGAGcGUGCGUgugggugggUGggugUGAGGGCcgGUGCg -3' miRNA: 3'- -UCUUcUACGCGa--------ACa---ACUUCCG--CACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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