Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29380 | 5' | -49.7 | NC_006151.1 | + | 44736 | 0.66 | 0.995071 |
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Target: 5'- aAGAAGccgagcAUGCGCgcgggUGUUGu--GUGUGCg -3' miRNA: 3'- -UCUUC------UACGCGa----ACAACuucCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 42988 | 0.66 | 0.995071 |
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Target: 5'- gAGAagAGAUGCG---GggGAGGGCGgcggGCg -3' miRNA: 3'- -UCU--UCUACGCgaaCaaCUUCCGCa---CG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 118422 | 0.66 | 0.99425 |
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Target: 5'- gAGGAGAcgacggGCGC-----GGAGGUGUGCg -3' miRNA: 3'- -UCUUCUa-----CGCGaacaaCUUCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 29805 | 0.67 | 0.993323 |
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Target: 5'- gGGGAGggGUGgUggugGUaGAGGGCGgugGCg -3' miRNA: 3'- -UCUUCuaCGCgAa---CAaCUUCCGCa--CG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 11730 | 0.67 | 0.993323 |
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Target: 5'- gGGggGAgcggGgGUgaGggGGAGGgGUGCg -3' miRNA: 3'- -UCuuCUa---CgCGaaCaaCUUCCgCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 46102 | 0.67 | 0.992279 |
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Target: 5'- gAGGAGGUGCGCcacgaGGAGGCcgaaggGCa -3' miRNA: 3'- -UCUUCUACGCGaacaaCUUCCGca----CG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 94509 | 0.67 | 0.992279 |
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Target: 5'- aGGAGGcccAUGaCGCg----GGAGGCGUGUa -3' miRNA: 3'- -UCUUC---UAC-GCGaacaaCUUCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 133203 | 1.14 | 0.004972 |
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Target: 5'- cAGAAGAUGCGCUUGUUGAAGGCGUGCg -3' miRNA: 3'- -UCUUCUACGCGAACAACUUCCGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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