Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 29380 | 5' | -49.7 | NC_006151.1 | + | 64284 | 0.69 | 0.973333 |
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Target: 5'- aAGAuGA-GCGCcgcGUUGucGGGCGUGCc -3' miRNA: 3'- -UCUuCUaCGCGaa-CAACu-UCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 125543 | 0.69 | 0.973333 |
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Target: 5'- uGGAGGUGCGC-----GgcGGCGUGUa -3' miRNA: 3'- uCUUCUACGCGaacaaCuuCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 23010 | 0.69 | 0.967198 |
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Target: 5'- gGGuGGggGCGCgggggUGgUGGAGGCG-GCg -3' miRNA: 3'- -UCuUCuaCGCGa----ACaACUUCCGCaCG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 90626 | 0.71 | 0.921636 |
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Target: 5'- cGAGGcggcccgcggGCGCUUGgcGAuGGGCGUGUa -3' miRNA: 3'- uCUUCua--------CGCGAACaaCU-UCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 8533 | 0.71 | 0.915633 |
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Target: 5'- gGGGAGA-GUGUgaUGUgGggGGUGUGCg -3' miRNA: 3'- -UCUUCUaCGCGa-ACAaCuuCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 27514 | 0.73 | 0.858309 |
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Target: 5'- gAGggGGUGCGa----UGggGGCGUGg -3' miRNA: 3'- -UCuuCUACGCgaacaACuuCCGCACg -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 30274 | 0.75 | 0.78655 |
|
Target: 5'- cAGAcAGcgGCGCggGggGAGGGUGUGUa -3' miRNA: 3'- -UCU-UCuaCGCGaaCaaCUUCCGCACG- -5' |
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| 29380 | 5' | -49.7 | NC_006151.1 | + | 133203 | 1.14 | 0.004972 |
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Target: 5'- cAGAAGAUGCGCUUGUUGAAGGCGUGCg -3' miRNA: 3'- -UCUUCUACGCGAACAACUUCCGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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