Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29448 | 5' | -50.1 | NC_006151.1 | + | 110656 | 0.67 | 0.988052 |
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Target: 5'- gGggGGACUUGggGgGauuGGGGCccaaagacgcggcgGUGGg -3' miRNA: 3'- aCuuCUUGAACuuCgU---CCCCGa-------------CAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 45030 | 0.67 | 0.987415 |
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Target: 5'- aGAAgcGAAUggggGAAGCguGGGGGgUGUGGg -3' miRNA: 3'- aCUU--CUUGaa--CUUCG--UCCCCgACAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 9550 | 0.67 | 0.987415 |
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Target: 5'- aGAAGAAacgggGAAGCGGGGGaaGg-- -3' miRNA: 3'- aCUUCUUgaa--CUUCGUCCCCgaCauc -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 49523 | 0.67 | 0.983829 |
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Target: 5'- cGGAGGACgaggagGggGgAGGGGg-GUGGg -3' miRNA: 3'- aCUUCUUGaa----CuuCgUCCCCgaCAUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 105020 | 0.67 | 0.981769 |
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Target: 5'- gGAGGAGCUggUGAcGCAGGcGGCg---- -3' miRNA: 3'- aCUUCUUGA--ACUuCGUCC-CCGacauc -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 96095 | 0.67 | 0.981769 |
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Target: 5'- cGAAGAGCaUG-AGC-GGGGUUGcGGg -3' miRNA: 3'- aCUUCUUGaACuUCGuCCCCGACaUC- -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 44781 | 0.68 | 0.977067 |
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Target: 5'- -aGAGAGCggggaGAAGCGGGGGgUGa-- -3' miRNA: 3'- acUUCUUGaa---CUUCGUCCCCgACauc -5' |
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| 29448 | 5' | -50.1 | NC_006151.1 | + | 72939 | 1.11 | 0.006616 |
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Target: 5'- gUGAAGAACUUGAAGCAGGGGCUGUAGg -3' miRNA: 3'- -ACUUCUUGAACUUCGUCCCCGACAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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