Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29485 | 5' | -53 | NC_006151.1 | + | 84673 | 0.7 | 0.842134 |
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Target: 5'- cGAGGCCGaGGUGGACgAGCAgcugCGGc -3' miRNA: 3'- -CUCCGGCaUCACCUG-UUGUagcaGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 88178 | 0.66 | 0.968239 |
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Target: 5'- cGAGGUgGUagacGGUGucGGCGGCGUgGUCGGa -3' miRNA: 3'- -CUCCGgCA----UCAC--CUGUUGUAgCAGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 90356 | 0.7 | 0.833754 |
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Target: 5'- uGAGGCUGgcGacgccGaGCAGCAUCGUCGAc -3' miRNA: 3'- -CUCCGGCauCa----CcUGUUGUAGCAGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 91719 | 0.66 | 0.971245 |
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Target: 5'- cGAGGCCGUcGUcGaacaGCGACAUCG-CGGc -3' miRNA: 3'- -CUCCGGCAuCAcC----UGUUGUAGCaGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 93801 | 0.74 | 0.627639 |
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Target: 5'- cGAGGCCGaGGuUGGAUGGCcUCGUCGc -3' miRNA: 3'- -CUCCGGCaUC-ACCUGUUGuAGCAGCu -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 97384 | 0.67 | 0.953584 |
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Target: 5'- cGGGGgcgucggccucguCCGUGccGUGGAUcGCGUUGUCGAc -3' miRNA: 3'- -CUCC-------------GGCAU--CACCUGuUGUAGCAGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 127540 | 0.69 | 0.901454 |
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Target: 5'- cGGGGCCcaGG-GGGCGuCGUCGUCGu -3' miRNA: 3'- -CUCCGGcaUCaCCUGUuGUAGCAGCu -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 129460 | 0.66 | 0.974041 |
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Target: 5'- -cGGCCGUGGgcgGGACGAgccCGUCc- -3' miRNA: 3'- cuCCGGCAUCa--CCUGUUguaGCAGcu -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 129690 | 0.66 | 0.96157 |
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Target: 5'- -cGGCCGccGUGGACcGCggCGcCGAg -3' miRNA: 3'- cuCCGGCauCACCUGuUGuaGCaGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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