Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29485 | 5' | -53 | NC_006151.1 | + | 76821 | 0.66 | 0.965016 |
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Target: 5'- cGGGCgCGUAGUGG-CGGCccgCGUCc- -3' miRNA: 3'- cUCCG-GCAUCACCuGUUGua-GCAGcu -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 64382 | 0.66 | 0.965016 |
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Target: 5'- cGAGGCCGUGGccagcccgcGGcGCAGCAgCGUCc- -3' miRNA: 3'- -CUCCGGCAUCa--------CC-UGUUGUaGCAGcu -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 88178 | 0.66 | 0.968239 |
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Target: 5'- cGAGGUgGUagacGGUGucGGCGGCGUgGUCGGa -3' miRNA: 3'- -CUCCGgCA----UCAC--CUGUUGUAgCAGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 6146 | 0.66 | 0.970365 |
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Target: 5'- -cGGCCGcgAGgacGGcggccucggccucgGCGGCGUCGUCGGa -3' miRNA: 3'- cuCCGGCa-UCa--CC--------------UGUUGUAGCAGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 27351 | 0.66 | 0.971245 |
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Target: 5'- cGGGGUCGUGGgccgGGGCccgGGCGg-GUCGAc -3' miRNA: 3'- -CUCCGGCAUCa---CCUG---UUGUagCAGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 91719 | 0.66 | 0.971245 |
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Target: 5'- cGAGGCCGUcGUcGaacaGCGACAUCG-CGGc -3' miRNA: 3'- -CUCCGGCAuCAcC----UGUUGUAGCaGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 129460 | 0.66 | 0.974041 |
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Target: 5'- -cGGCCGUGGgcgGGACGAgccCGUCc- -3' miRNA: 3'- cuCCGGCAUCa--CCUGUUguaGCAGcu -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 64555 | 0.66 | 0.974041 |
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Target: 5'- cGAGGCCGUGGcGcGcCAGC-UCGaCGAg -3' miRNA: 3'- -CUCCGGCAUCaC-CuGUUGuAGCaGCU- -5' |
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| 29485 | 5' | -53 | NC_006151.1 | + | 39501 | 0.66 | 0.976635 |
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Target: 5'- -cGGCCGUGcUGGACucuagcucCAUCGUgGGc -3' miRNA: 3'- cuCCGGCAUcACCUGuu------GUAGCAgCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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