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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29553 | 5' | -59.3 | NC_006151.1 | + | 27200 | 0.66 | 0.687701 |
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Target: 5'- -cCGCGcagaaggcGCCGggGCcccGCGCGCCGUGGa -3' miRNA: 3'- aaGCGC--------UGGCa-CGa--CGCGCGGUACUa -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 132170 | 0.66 | 0.687701 |
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Target: 5'- -gCGCGGCCG-GCaccgGCGCGCCc---- -3' miRNA: 3'- aaGCGCUGGCaCGa---CGCGCGGuacua -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 103745 | 0.66 | 0.687701 |
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Target: 5'- -gCGCGGCaGUGCgaggaGCGCGCCGcGGc -3' miRNA: 3'- aaGCGCUGgCACGa----CGCGCGGUaCUa -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 49616 | 0.66 | 0.687701 |
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Target: 5'- gUCcUGGCgGUGCUGCGCgaguggggguggGCCGUGGa -3' miRNA: 3'- aAGcGCUGgCACGACGCG------------CGGUACUa -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 106128 | 0.66 | 0.696806 |
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Target: 5'- -aCGCGGCCGUGCUGgccacgacgcucuCGCGCa----- -3' miRNA: 3'- aaGCGCUGGCACGAC-------------GCGCGguacua -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 143010 | 0.66 | 0.703858 |
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Target: 5'- aUCGCGGCuCGccuucuaggguugGCUGUGUGCCAagGAUg -3' miRNA: 3'- aAGCGCUG-GCa------------CGACGCGCGGUa-CUA- -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 104883 | 0.66 | 0.707874 |
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Target: 5'- -aCGCG-CC-UGCUGCGCGagGUGGUg -3' miRNA: 3'- aaGCGCuGGcACGACGCGCggUACUA- -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 119086 | 0.66 | 0.707874 |
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Target: 5'- cUCGCaGGCCGcgGCgcucgGCGCGUaCGUGAg -3' miRNA: 3'- aAGCG-CUGGCa-CGa----CGCGCG-GUACUa -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 137677 | 0.66 | 0.707874 |
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Target: 5'- -cCGcCGugCG-GCUGcCGCGCCAaGAUg -3' miRNA: 3'- aaGC-GCugGCaCGAC-GCGCGGUaCUA- -5' |
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| 29553 | 5' | -59.3 | NC_006151.1 | + | 64298 | 0.66 | 0.717868 |
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Target: 5'- gUUGuCGGgCGUGCcgcaGCGCGCCGUGc- -3' miRNA: 3'- aAGC-GCUgGCACGa---CGCGCGGUACua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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