Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29613 | 5' | -51.9 | NC_006151.1 | + | 103680 | 0.68 | 0.925154 |
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Target: 5'- cGCGC-GCGGcggGGGUGCUGCGcGCc- -3' miRNA: 3'- -CGCGaCGUCauaUCUAUGACGC-CGac -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 111277 | 0.7 | 0.879372 |
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Target: 5'- cGCGCggGCGGg--GGGUAC-GCGGcCUGg -3' miRNA: 3'- -CGCGa-CGUCauaUCUAUGaCGCC-GAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 103224 | 0.7 | 0.879372 |
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Target: 5'- cGCGCgUGCGcgcgAUGGAggaccUGCUGCGGCa- -3' miRNA: 3'- -CGCG-ACGUca--UAUCU-----AUGACGCCGac -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 5699 | 0.7 | 0.879372 |
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Target: 5'- gGCGCUGCGGccacc--GCUGCuGGCUGu -3' miRNA: 3'- -CGCGACGUCauaucuaUGACG-CCGAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 104290 | 0.7 | 0.871836 |
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Target: 5'- cCGCUGCAGgc--GcUGCaGCGGCUGa -3' miRNA: 3'- cGCGACGUCauauCuAUGaCGCCGAC- -5' |
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| 29613 | 5' | -51.9 | NC_006151.1 | + | 61314 | 0.72 | 0.7653 |
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Target: 5'- cGCGCUGCAGguccagGGAgACgcccggccaGCGGCUGc -3' miRNA: 3'- -CGCGACGUCaua---UCUaUGa--------CGCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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