Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30404 | 5' | -58.8 | NC_006548.1 | + | 27169 | 0.69 | 0.238872 |
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Target: 5'- -cCGCUUGCacagcagcauugaGGCGCUgCGUACUGGCUg -3' miRNA: 3'- uuGCGAAUGg------------UCGCGG-GCAUGGCCGA- -5' |
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| 30404 | 5' | -58.8 | NC_006548.1 | + | 28559 | 0.66 | 0.388796 |
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Target: 5'- cGGCGC-UACCcGCGacuaCCGUcACCGGUa -3' miRNA: 3'- -UUGCGaAUGGuCGCg---GGCA-UGGCCGa -5' |
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| 30404 | 5' | -58.8 | NC_006548.1 | + | 32150 | 0.69 | 0.254437 |
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Target: 5'- --gGCUcgGCCAGCGCCuCGaccuugGCUGGCa -3' miRNA: 3'- uugCGAa-UGGUCGCGG-GCa-----UGGCCGa -5' |
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| 30404 | 5' | -58.8 | NC_006548.1 | + | 32856 | 0.77 | 0.065954 |
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Target: 5'- uGACGCUUGCCAGCagcuggauGUCCGgccacACCGGCa -3' miRNA: 3'- -UUGCGAAUGGUCG--------CGGGCa----UGGCCGa -5' |
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| 30404 | 5' | -58.8 | NC_006548.1 | + | 35867 | 0.66 | 0.398015 |
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Target: 5'- uGCGaagaaUGCCGGCGCgauCCGcgcgGCCGGCg -3' miRNA: 3'- uUGCga---AUGGUCGCG---GGCa---UGGCCGa -5' |
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| 30404 | 5' | -58.8 | NC_006548.1 | + | 36620 | 0.7 | 0.228943 |
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Target: 5'- gGAUGCgucgauacggUGCCAGCGCCgCaugagGCCGGCg -3' miRNA: 3'- -UUGCGa---------AUGGUCGCGG-Gca---UGGCCGa -5' |
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| 30404 | 5' | -58.8 | NC_006548.1 | + | 37712 | 0.67 | 0.344851 |
|
Target: 5'- cACGCUcaucaCAGUGCCgGcgACCGGCg -3' miRNA: 3'- uUGCGAaug--GUCGCGGgCa-UGGCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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