Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30404 | 5' | -58.8 | NC_006548.1 | + | 23412 | 0.66 | 0.379718 |
Target: 5'- uAACGCUUgGCCAGUGgcagaCUGUAgccCCGGCg -3' miRNA: 3'- -UUGCGAA-UGGUCGCg----GGCAU---GGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 2368 | 0.66 | 0.379718 |
Target: 5'- -cCGCcagGCCGGCGguuuuccaUCCGaGCCGGCUg -3' miRNA: 3'- uuGCGaa-UGGUCGC--------GGGCaUGGCCGA- -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 12810 | 0.66 | 0.388796 |
Target: 5'- -uUGCUcGCCAGUGCCgCGcgAgCGGCg -3' miRNA: 3'- uuGCGAaUGGUCGCGG-GCa-UgGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 28559 | 0.66 | 0.388796 |
Target: 5'- cGGCGC-UACCcGCGacuaCCGUcACCGGUa -3' miRNA: 3'- -UUGCGaAUGGuCGCg---GGCA-UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 35867 | 0.66 | 0.398015 |
Target: 5'- uGCGaagaaUGCCGGCGCgauCCGcgcgGCCGGCg -3' miRNA: 3'- uUGCga---AUGGUCGCG---GGCa---UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 1539 | 0.66 | 0.398015 |
Target: 5'- gAGCGCUaccacuccacUGCCGGCGaCCGccUCGGCg -3' miRNA: 3'- -UUGCGA----------AUGGUCGCgGGCauGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 21981 | 0.66 | 0.407373 |
Target: 5'- uAGCGUcauCCuGCGCCCGaacAUCGGCg -3' miRNA: 3'- -UUGCGaauGGuCGCGGGCa--UGGCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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