Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30404 | 5' | -58.8 | NC_006548.1 | + | 36620 | 0.7 | 0.228943 |
Target: 5'- gGAUGCgucgauacggUGCCAGCGCCgCaugagGCCGGCg -3' miRNA: 3'- -UUGCGa---------AUGGUCGCGG-Gca---UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 22719 | 0.7 | 0.222916 |
Target: 5'- aGGCGCagaa-GGUGCUCGUGCCGGCc -3' miRNA: 3'- -UUGCGaauggUCGCGGGCAUGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 7253 | 0.7 | 0.205642 |
Target: 5'- uGCGCUgccccugACCGGCGCuCUGUagcuGCCGGUc -3' miRNA: 3'- uUGCGAa------UGGUCGCG-GGCA----UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 22026 | 0.7 | 0.200147 |
Target: 5'- uGACGCUauCCAGCGCaUCGgugaucACCGGCa -3' miRNA: 3'- -UUGCGAauGGUCGCG-GGCa-----UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 22331 | 0.71 | 0.174554 |
Target: 5'- uGCGCggugGCCAGCGCguCCGUGUCGGUg -3' miRNA: 3'- uUGCGaa--UGGUCGCG--GGCAUGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 32856 | 0.77 | 0.065954 |
Target: 5'- uGACGCUUGCCAGCagcuggauGUCCGgccacACCGGCa -3' miRNA: 3'- -UUGCGAAUGGUCG--------CGGGCa----UGGCCGa -5' |
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30404 | 5' | -58.8 | NC_006548.1 | + | 1276 | 1.04 | 0.00051 |
Target: 5'- cAACGCUUACCAGCGCCCGUACCGGCUu -3' miRNA: 3'- -UUGCGAAUGGUCGCGGGCAUGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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