miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30404 5' -58.8 NC_006548.1 + 36620 0.7 0.228943
Target:  5'- gGAUGCgucgauacggUGCCAGCGCCgCaugagGCCGGCg -3'
miRNA:   3'- -UUGCGa---------AUGGUCGCGG-Gca---UGGCCGa -5'
30404 5' -58.8 NC_006548.1 + 22719 0.7 0.222916
Target:  5'- aGGCGCagaa-GGUGCUCGUGCCGGCc -3'
miRNA:   3'- -UUGCGaauggUCGCGGGCAUGGCCGa -5'
30404 5' -58.8 NC_006548.1 + 7253 0.7 0.205642
Target:  5'- uGCGCUgccccugACCGGCGCuCUGUagcuGCCGGUc -3'
miRNA:   3'- uUGCGAa------UGGUCGCG-GGCA----UGGCCGa -5'
30404 5' -58.8 NC_006548.1 + 22026 0.7 0.200147
Target:  5'- uGACGCUauCCAGCGCaUCGgugaucACCGGCa -3'
miRNA:   3'- -UUGCGAauGGUCGCG-GGCa-----UGGCCGa -5'
30404 5' -58.8 NC_006548.1 + 22331 0.71 0.174554
Target:  5'- uGCGCggugGCCAGCGCguCCGUGUCGGUg -3'
miRNA:   3'- uUGCGaa--UGGUCGCG--GGCAUGGCCGa -5'
30404 5' -58.8 NC_006548.1 + 32856 0.77 0.065954
Target:  5'- uGACGCUUGCCAGCagcuggauGUCCGgccacACCGGCa -3'
miRNA:   3'- -UUGCGAAUGGUCG--------CGGGCa----UGGCCGa -5'
30404 5' -58.8 NC_006548.1 + 1276 1.04 0.00051
Target:  5'- cAACGCUUACCAGCGCCCGUACCGGCUu -3'
miRNA:   3'- -UUGCGAAUGGUCGCGGGCAUGGCCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.