Results 21 - 37 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30407 | 3' | -57.9 | NC_006548.1 | + | 20090 | 0.68 | 0.352074 |
Target: 5'- aGCCgcACCAGGUGgUgccGGAGUucgccgaGGCGGCg -3' miRNA: 3'- gCGGa-UGGUCCACgA---CCUCG-------CUGUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 17613 | 0.68 | 0.361533 |
Target: 5'- gGCCaugGCCgAGGgGCUGGAG--GCAGCu -3' miRNA: 3'- gCGGa--UGG-UCCaCGACCUCgcUGUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 15200 | 0.68 | 0.365016 |
Target: 5'- gGCCUGcgcguCCGGGuUGCUGGAcauacuguggauacgGCGGCcauuGGCg -3' miRNA: 3'- gCGGAU-----GGUCC-ACGACCU---------------CGCUG----UCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 25793 | 0.68 | 0.369403 |
Target: 5'- uGCCUguucggcACCAGGUaCUGGAGUuGCcGCu -3' miRNA: 3'- gCGGA-------UGGUCCAcGACCUCGcUGuCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 4765 | 0.67 | 0.388217 |
Target: 5'- gCGCU--UCAGGUGCUuGAGCGuggcgauCGGCa -3' miRNA: 3'- -GCGGauGGUCCACGAcCUCGCu------GUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 30417 | 0.67 | 0.401104 |
Target: 5'- gCGCCUGauGGGcggcacggcguucaaUGCUGGGGCGAUcccgcccgguGGCg -3' miRNA: 3'- -GCGGAUggUCC---------------ACGACCUCGCUG----------UCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 35930 | 0.67 | 0.405773 |
Target: 5'- gGCCUACCGaccGGcgaGUUGGccggcagGGUGGCGGCg -3' miRNA: 3'- gCGGAUGGU---CCa--CGACC-------UCGCUGUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 15231 | 0.67 | 0.40671 |
Target: 5'- uGCCUAuCCGGGcaucacCUGGGGUGGC-GCu -3' miRNA: 3'- gCGGAU-GGUCCac----GACCUCGCUGuCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 29609 | 0.67 | 0.425747 |
Target: 5'- cCGCCUGCgGuGGaaagcgcuccUGCggcGGAgGUGACAGCa -3' miRNA: 3'- -GCGGAUGgU-CC----------ACGa--CCU-CGCUGUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 12152 | 0.67 | 0.425747 |
Target: 5'- cCGCCgGCCAGcaGCaGGAGCauCAGCg -3' miRNA: 3'- -GCGGaUGGUCcaCGaCCUCGcuGUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 33122 | 0.66 | 0.445304 |
Target: 5'- uGCCcucgacaacaUACCGGG-GCUGGccGGUgcucgGGCAGCg -3' miRNA: 3'- gCGG----------AUGGUCCaCGACC--UCG-----CUGUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 37254 | 0.66 | 0.455269 |
Target: 5'- gGCCUGCCGGGaGaaGG-GCauuACAGCg -3' miRNA: 3'- gCGGAUGGUCCaCgaCCuCGc--UGUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 22299 | 0.66 | 0.455269 |
Target: 5'- uGCCgUugCGGGUGUcGGccaGGCGAUcGCg -3' miRNA: 3'- gCGG-AugGUCCACGaCC---UCGCUGuCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 24018 | 0.66 | 0.455269 |
Target: 5'- gCGCCUGCCAGGccg-GGcGGUGucCAGCc -3' miRNA: 3'- -GCGGAUGGUCCacgaCC-UCGCu-GUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 3849 | 0.66 | 0.465354 |
Target: 5'- gGUCUACC---UGCaGGAGCGGCGcGCu -3' miRNA: 3'- gCGGAUGGuccACGaCCUCGCUGU-CG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 6083 | 0.66 | 0.475553 |
Target: 5'- uCGC--GCCAGGgGUUGcGGGUGAuCAGCa -3' miRNA: 3'- -GCGgaUGGUCCaCGAC-CUCGCU-GUCG- -5' |
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30407 | 3' | -57.9 | NC_006548.1 | + | 1200 | 0.66 | 0.475553 |
Target: 5'- uCGCCgcaCAGGcguUGCUGGccgcGCaGACGGCc -3' miRNA: 3'- -GCGGaugGUCC---ACGACCu---CG-CUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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