Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30407 | 5' | -53.5 | NC_006548.1 | + | 9051 | 0.66 | 0.685592 |
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Target: 5'- uGUGCAuccauugAGGGCUg-CGUggagcacGGUACGCAGAg -3' miRNA: 3'- -CGCGU-------UCCUGAagGCA-------UCGUGCGUCU- -5' |
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| 30407 | 5' | -53.5 | NC_006548.1 | + | 32581 | 0.67 | 0.653689 |
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Target: 5'- uGCGCc-GGACUgggCUGaUGGCGUGCAGGa -3' miRNA: 3'- -CGCGuuCCUGAa--GGC-AUCGUGCGUCU- -5' |
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| 30407 | 5' | -53.5 | NC_006548.1 | + | 19140 | 0.67 | 0.642241 |
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Target: 5'- aGCGCuGGGAgaUCCG-AGCGggcuucUGCAGGa -3' miRNA: 3'- -CGCGuUCCUgaAGGCaUCGU------GCGUCU- -5' |
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| 30407 | 5' | -53.5 | NC_006548.1 | + | 29026 | 0.67 | 0.630783 |
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Target: 5'- aGCGCcuucGGugUcacCCGUGGCGaGCAGAc -3' miRNA: 3'- -CGCGuu--CCugAa--GGCAUCGUgCGUCU- -5' |
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| 30407 | 5' | -53.5 | NC_006548.1 | + | 33712 | 0.68 | 0.596459 |
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Target: 5'- -aGCAcGGugUUCCGaggAGUACGCGa- -3' miRNA: 3'- cgCGUuCCugAAGGCa--UCGUGCGUcu -5' |
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| 30407 | 5' | -53.5 | NC_006548.1 | + | 35003 | 0.69 | 0.496219 |
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Target: 5'- -aGCAAuGGGCggCCGUAGCaaaGCGCAa- -3' miRNA: 3'- cgCGUU-CCUGaaGGCAUCG---UGCGUcu -5' |
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| 30407 | 5' | -53.5 | NC_006548.1 | + | 2248 | 0.97 | 0.006798 |
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Target: 5'- cGCGCAAGGACUUCCugAGCACGCAGAa -3' miRNA: 3'- -CGCGUUCCUGAAGGcaUCGUGCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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