Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30413 | 3' | -58.5 | NC_006548.1 | + | 35891 | 0.69 | 0.274853 |
Target: 5'- -cGCgGCCGGCGG-CGUGAUAAcGACGAc -3' miRNA: 3'- cuCG-UGGUCGCCgGCGCUGUU-CUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 28542 | 0.7 | 0.254389 |
Target: 5'- cGGCGCCGGCcaGGUCGCGGCGcuacccGCGAc -3' miRNA: 3'- cUCGUGGUCG--CCGGCGCUGUuc----UGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 17964 | 0.7 | 0.254389 |
Target: 5'- cAGC-CUGGCGGgCGCGAgugaucuaCGAGACGAa -3' miRNA: 3'- cUCGuGGUCGCCgGCGCU--------GUUCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 36651 | 0.7 | 0.229071 |
Target: 5'- cGAGCACCGcgguGcCGGCCGCGGCGuuGAUc- -3' miRNA: 3'- -CUCGUGGU----C-GCCGGCGCUGUu-CUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 13393 | 0.7 | 0.231504 |
Target: 5'- uGAGCGCCGGCGGaaucggcgcgcgcucCUGCGGC-GGGCa- -3' miRNA: 3'- -CUCGUGGUCGCC---------------GGCGCUGuUCUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 11733 | 0.71 | 0.195113 |
Target: 5'- cGGCGCCGG-GGuuGCGGCGAugacauGACGAu -3' miRNA: 3'- cUCGUGGUCgCCggCGCUGUU------CUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 2922 | 0.71 | 0.217229 |
Target: 5'- cGAGCGCCugcaGGCGGCCaaGGCGcugaucGACGAg -3' miRNA: 3'- -CUCGUGG----UCGCCGGcgCUGUu-----CUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 37544 | 0.72 | 0.170259 |
Target: 5'- cGGCGCCGGCGGuuG-GGCuGAGugGAc -3' miRNA: 3'- cUCGUGGUCGCCggCgCUG-UUCugCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 20031 | 0.72 | 0.174992 |
Target: 5'- uGAGCGCCGGaucguaGGUCGCGGCGaucuccugcaGGAUGGc -3' miRNA: 3'- -CUCGUGGUCg-----CCGGCGCUGU----------UCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 18792 | 0.73 | 0.140227 |
Target: 5'- cGAGCAgCA-CGGCCuGCGGCuGGGCGAg -3' miRNA: 3'- -CUCGUgGUcGCCGG-CGCUGuUCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 29906 | 0.73 | 0.15674 |
Target: 5'- cGGCGCUGuGCGGCaCGCGAC-GGGCGGc -3' miRNA: 3'- cUCGUGGU-CGCCG-GCGCUGuUCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 4551 | 1.08 | 0.000319 |
Target: 5'- uGAGCACCAGCGGCCGCGACAAGACGAg -3' miRNA: 3'- -CUCGUGGUCGCCGGCGCUGUUCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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