Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30413 | 3' | -58.5 | NC_006548.1 | + | 1965 | 0.68 | 0.335806 |
Target: 5'- cGGGCACCA--GGCCGUGGCucGGCu- -3' miRNA: 3'- -CUCGUGGUcgCCGGCGCUGuuCUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 7816 | 0.68 | 0.344084 |
Target: 5'- uGAGCugCcGCGaGCUGCG-CAAGGCu- -3' miRNA: 3'- -CUCGugGuCGC-CGGCGCuGUUCUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 16406 | 0.68 | 0.34492 |
Target: 5'- uGGgGCCAGCGGCaauuggccguagucaGCcGCGAGugGAa -3' miRNA: 3'- cUCgUGGUCGCCGg--------------CGcUGUUCugCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 33862 | 0.67 | 0.361077 |
Target: 5'- uAGguCCAGCGGaUCGgGcCGAGGCGGa -3' miRNA: 3'- cUCguGGUCGCC-GGCgCuGUUCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 21434 | 0.67 | 0.368913 |
Target: 5'- uGGCgGCCGGCGGCagaguCGCGGCAaccgcucgccccuGGGCGu -3' miRNA: 3'- cUCG-UGGUCGCCG-----GCGCUGU-------------UCUGCu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 28888 | 0.67 | 0.384929 |
Target: 5'- cGGGcCACCggccaaaccgucgaGGCGGCCGgCGcCGAGAuCGAg -3' miRNA: 3'- -CUC-GUGG--------------UCGCCGGC-GCuGUUCU-GCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 1128 | 0.67 | 0.387643 |
Target: 5'- -uGCGCCGGCugGGUCGuCGACAuGGCa- -3' miRNA: 3'- cuCGUGGUCG--CCGGC-GCUGUuCUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 14258 | 0.67 | 0.387643 |
Target: 5'- gGGGCGCCuGGCcgauGGCCGgaUGACccuGGACGAg -3' miRNA: 3'- -CUCGUGG-UCG----CCGGC--GCUGu--UCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 17444 | 0.67 | 0.39678 |
Target: 5'- cGGCGCC-GUGGCCuCGGCcuGGGugGAc -3' miRNA: 3'- cUCGUGGuCGCCGGcGCUG--UUCugCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 26329 | 0.66 | 0.415463 |
Target: 5'- aGAGCGCguuCGGCGGCCGgGuCGAuGGCc- -3' miRNA: 3'- -CUCGUG---GUCGCCGGCgCuGUU-CUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 16261 | 0.66 | 0.415463 |
Target: 5'- -cGcCACCAGUggcgacGGCCGCGACAucacuCGAc -3' miRNA: 3'- cuC-GUGGUCG------CCGGCGCUGUucu--GCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 33194 | 0.66 | 0.425004 |
Target: 5'- -cGUACCAGCGGCUGCucgauguccugGAUA--ACGAa -3' miRNA: 3'- cuCGUGGUCGCCGGCG-----------CUGUucUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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