Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30413 | 3' | -58.5 | NC_006548.1 | + | 26329 | 0.66 | 0.415463 |
Target: 5'- aGAGCGCguuCGGCGGCCGgGuCGAuGGCc- -3' miRNA: 3'- -CUCGUG---GUCGCCGGCgCuGUU-CUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 28542 | 0.7 | 0.254389 |
Target: 5'- cGGCGCCGGCcaGGUCGCGGCGcuacccGCGAc -3' miRNA: 3'- cUCGUGGUCG--CCGGCGCUGUuc----UGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 28888 | 0.67 | 0.384929 |
Target: 5'- cGGGcCACCggccaaaccgucgaGGCGGCCGgCGcCGAGAuCGAg -3' miRNA: 3'- -CUC-GUGG--------------UCGCCGGC-GCuGUUCU-GCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 29604 | 0.68 | 0.31185 |
Target: 5'- gGAGCcgcaGCCAgucuuGCGGCCGCGAauGGGCu- -3' miRNA: 3'- -CUCG----UGGU-----CGCCGGCGCUguUCUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 29654 | 0.69 | 0.274853 |
Target: 5'- -cGCuGCCAGCGGUgccggUGCGGCAGGuGCGGc -3' miRNA: 3'- cuCG-UGGUCGCCG-----GCGCUGUUC-UGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 29906 | 0.73 | 0.15674 |
Target: 5'- cGGCGCUGuGCGGCaCGCGAC-GGGCGGc -3' miRNA: 3'- cUCGUGGU-CGCCG-GCGCUGuUCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 33194 | 0.66 | 0.425004 |
Target: 5'- -cGUACCAGCGGCUGCucgauguccugGAUA--ACGAa -3' miRNA: 3'- cuCGUGGUCGCCGGCG-----------CUGUucUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 33862 | 0.67 | 0.361077 |
Target: 5'- uAGguCCAGCGGaUCGgGcCGAGGCGGa -3' miRNA: 3'- cUCguGGUCGCC-GGCgCuGUUCUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 35486 | 0.69 | 0.268579 |
Target: 5'- cGAGUGCCAGaccaaccgcgacuggGGCCGUGGCAAGgaucGCGGc -3' miRNA: 3'- -CUCGUGGUCg--------------CCGGCGCUGUUC----UGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 35891 | 0.69 | 0.274853 |
Target: 5'- -cGCgGCCGGCGG-CGUGAUAAcGACGAc -3' miRNA: 3'- cuCG-UGGUCGCCgGCGCUGUU-CUGCU- -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 36651 | 0.7 | 0.229071 |
Target: 5'- cGAGCACCGcgguGcCGGCCGCGGCGuuGAUc- -3' miRNA: 3'- -CUCGUGGU----C-GCCGGCGCUGUu-CUGcu -5' |
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30413 | 3' | -58.5 | NC_006548.1 | + | 37544 | 0.72 | 0.170259 |
Target: 5'- cGGCGCCGGCGGuuG-GGCuGAGugGAc -3' miRNA: 3'- cUCGUGGUCGCCggCgCUG-UUCugCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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