Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30418 | 5' | -54.8 | NC_006548.1 | + | 30515 | 0.69 | 0.475734 |
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Target: 5'- uGGCAUGGAAC-CAGCcgau--GCCCUa -3' miRNA: 3'- -CCGUGCCUUGuGUCGaaccauCGGGG- -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 30718 | 0.7 | 0.42516 |
|
Target: 5'- cGGCAcCGGGGCAuCAGC-----AGCCCCu -3' miRNA: 3'- -CCGU-GCCUUGU-GUCGaaccaUCGGGG- -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 31223 | 0.66 | 0.641043 |
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Target: 5'- uGCGUGGAcGCGCAGCUUGGcgAGCa-- -3' miRNA: 3'- cCGUGCCU-UGUGUCGAACCa-UCGggg -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 34459 | 0.66 | 0.641043 |
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Target: 5'- aGUACcaGGAGCaACAGCgaGGUGccGCCCa -3' miRNA: 3'- cCGUG--CCUUG-UGUCGaaCCAU--CGGGg -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 34911 | 0.69 | 0.463316 |
|
Target: 5'- cGCAcCGGAuACGCAGUaacccggccggcUGGUcAGCCCCg -3' miRNA: 3'- cCGU-GCCU-UGUGUCGa-----------ACCA-UCGGGG- -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 35015 | 0.74 | 0.22023 |
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Target: 5'- aGGCGCaGGGC-CAGCUcgucaccGGUAGCCUCa -3' miRNA: 3'- -CCGUGcCUUGuGUCGAa------CCAUCGGGG- -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 36197 | 0.66 | 0.629723 |
|
Target: 5'- aGCGCGGug-AUGGCgaUGGU-GCCCCu -3' miRNA: 3'- cCGUGCCuugUGUCGa-ACCAuCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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