Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30418 | 5' | -54.8 | NC_006548.1 | + | 34911 | 0.69 | 0.463316 |
Target: 5'- cGCAcCGGAuACGCAGUaacccggccggcUGGUcAGCCCCg -3' miRNA: 3'- cCGU-GCCU-UGUGUCGa-----------ACCA-UCGGGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 18453 | 0.7 | 0.387092 |
Target: 5'- cGGUuCGGGugGCAGCggcgUGGUcuucugcagGGCgCCCu -3' miRNA: 3'- -CCGuGCCUugUGUCGa---ACCA---------UCG-GGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 14406 | 0.71 | 0.334361 |
Target: 5'- uGCACGGcuGCACGGCUgGGcAGCUCg -3' miRNA: 3'- cCGUGCCu-UGUGUCGAaCCaUCGGGg -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 20712 | 0.72 | 0.294582 |
Target: 5'- uGGCGCuccuGGAGCGCAGCUucgucaggcaUGGUcguGUCCUg -3' miRNA: 3'- -CCGUG----CCUUGUGUCGA----------ACCAu--CGGGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 35015 | 0.74 | 0.22023 |
Target: 5'- aGGCGCaGGGC-CAGCUcgucaccGGUAGCCUCa -3' miRNA: 3'- -CCGUGcCUUGuGUCGAa------CCAUCGGGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 14483 | 0.81 | 0.081237 |
Target: 5'- gGGCACGGAu--CAGCUUGGccGCCUCg -3' miRNA: 3'- -CCGUGCCUuguGUCGAACCauCGGGG- -5' |
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30418 | 5' | -54.8 | NC_006548.1 | + | 5817 | 1.14 | 0.000333 |
Target: 5'- cGGCACGGAACACAGCUUGGUAGCCCCc -3' miRNA: 3'- -CCGUGCCUUGUGUCGAACCAUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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