Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30418 | 5' | -54.8 | NC_006548.1 | + | 20903 | 0.67 | 0.595818 |
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Target: 5'- cGGCcgaGGugcuGCGCAGCUUGcUGGCCg- -3' miRNA: 3'- -CCGug-CCu---UGUGUCGAACcAUCGGgg -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 6857 | 0.66 | 0.6071 |
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Target: 5'- uGGUuCGGAugcaggcuGCGCAGUUcGGUgaccGGCUCCg -3' miRNA: 3'- -CCGuGCCU--------UGUGUCGAaCCA----UCGGGG- -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 36197 | 0.66 | 0.629723 |
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Target: 5'- aGCGCGGug-AUGGCgaUGGU-GCCCCu -3' miRNA: 3'- cCGUGCCuugUGUCGa-ACCAuCGGGG- -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 34459 | 0.66 | 0.641043 |
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Target: 5'- aGUACcaGGAGCaACAGCgaGGUGccGCCCa -3' miRNA: 3'- cCGUG--CCUUG-UGUCGaaCCAU--CGGGg -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 31223 | 0.66 | 0.641043 |
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Target: 5'- uGCGUGGAcGCGCAGCUUGGcgAGCa-- -3' miRNA: 3'- cCGUGCCU-UGUGUCGAACCa-UCGggg -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 19028 | 0.66 | 0.641043 |
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Target: 5'- uGGCGCcu-GCACGGUggaGGUGGCgUCCa -3' miRNA: 3'- -CCGUGccuUGUGUCGaa-CCAUCG-GGG- -5' |
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| 30418 | 5' | -54.8 | NC_006548.1 | + | 28826 | 0.66 | 0.663645 |
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Target: 5'- cGGCGC-GAGCACgGGCUUccagGGCUCCu -3' miRNA: 3'- -CCGUGcCUUGUG-UCGAAcca-UCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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