Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30422 | 3' | -53.1 | NC_006548.1 | + | 32437 | 0.67 | 0.633564 |
Target: 5'- --cGCgGCGcUGCAGGAgCGCAUCugGACu -3' miRNA: 3'- aguCGgCGU-ACGUCUUgGCGUAG--UUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 34088 | 0.7 | 0.464448 |
Target: 5'- aCAGCCGgGUGCAGAucaaaugccgGCUGC-UCggUa -3' miRNA: 3'- aGUCGGCgUACGUCU----------UGGCGuAGuuG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 4898 | 0.7 | 0.464448 |
Target: 5'- uUCGGCCGCuccagugGCGcAACCGCAgccaucauggcuUCGGCa -3' miRNA: 3'- -AGUCGGCGua-----CGUcUUGGCGU------------AGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 30857 | 0.69 | 0.507738 |
Target: 5'- gCGGCCGuUAUGCGGGcagcagugaaGCCGUugucaAUCAGCu -3' miRNA: 3'- aGUCGGC-GUACGUCU----------UGGCG-----UAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 36392 | 0.69 | 0.530019 |
Target: 5'- cUCAGCCGCGUG-GGAuCUGCAcgguUCGAa -3' miRNA: 3'- -AGUCGGCGUACgUCUuGGCGU----AGUUg -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 24457 | 0.68 | 0.541293 |
Target: 5'- -uGGCCGCuUGCAGcgcggcaaGACCGCcacuAUUAACa -3' miRNA: 3'- agUCGGCGuACGUC--------UUGGCG----UAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 20538 | 0.68 | 0.541293 |
Target: 5'- --cGCCGC-UGCGGAagaGCCGCAg-GGCa -3' miRNA: 3'- aguCGGCGuACGUCU---UGGCGUagUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 4300 | 0.67 | 0.610287 |
Target: 5'- gUCAGCaCG-GUGCAGAGCgGCAgaugggUAGCu -3' miRNA: 3'- -AGUCG-GCgUACGUCUUGgCGUa-----GUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 12586 | 0.67 | 0.633564 |
Target: 5'- cUCGGCCGCcaacuuccGCgAGAACCGCAa---- -3' miRNA: 3'- -AGUCGGCGua------CG-UCUUGGCGUaguug -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 33656 | 0.7 | 0.453926 |
Target: 5'- aCAGCgacaGCGUGCAGAucacccacgaaACCgacaccgacgGCAUCAGCa -3' miRNA: 3'- aGUCGg---CGUACGUCU-----------UGG----------CGUAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 18430 | 0.7 | 0.453926 |
Target: 5'- cCAGCaGCuucUGCAGAACCGC-UCggUg -3' miRNA: 3'- aGUCGgCGu--ACGUCUUGGCGuAGuuG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 18733 | 0.7 | 0.433279 |
Target: 5'- cCAGCCGCAgGCcGuGCUGC-UCGACg -3' miRNA: 3'- aGUCGGCGUaCGuCuUGGCGuAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 22543 | 0.74 | 0.274746 |
Target: 5'- aCAGCCGCcgGCGGAggagaGCCugAUCGACg -3' miRNA: 3'- aGUCGGCGuaCGUCU-----UGGcgUAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 38228 | 0.73 | 0.313616 |
Target: 5'- cUCGGCCGauucuUGCGGGAggGCAUCGGCa -3' miRNA: 3'- -AGUCGGCgu---ACGUCUUggCGUAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 36541 | 0.73 | 0.321872 |
Target: 5'- cUCGGCCGCGaugaucgaUGacgAGAucuuCCGCGUCGACg -3' miRNA: 3'- -AGUCGGCGU--------ACg--UCUu---GGCGUAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 7006 | 0.71 | 0.374784 |
Target: 5'- aUCaAGCCGCuggcGCgaAGAuccucgACCGCAUCAGCa -3' miRNA: 3'- -AG-UCGGCGua--CG--UCU------UGGCGUAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 26986 | 0.71 | 0.393682 |
Target: 5'- -uGGCCGUAaGCuGAGCaGCGUCGGCg -3' miRNA: 3'- agUCGGCGUaCGuCUUGgCGUAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 36470 | 0.71 | 0.413188 |
Target: 5'- aUCGGCCGgG-GCcuGACCaGCGUCAGCg -3' miRNA: 3'- -AGUCGGCgUaCGucUUGG-CGUAGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 33299 | 0.7 | 0.43226 |
Target: 5'- cCGGCCGCGaggagcaUGCGGcGCUGCG-CAGCc -3' miRNA: 3'- aGUCGGCGU-------ACGUCuUGGCGUaGUUG- -5' |
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30422 | 3' | -53.1 | NC_006548.1 | + | 22952 | 0.7 | 0.433279 |
Target: 5'- -uGGCCGCcgGCcucaAGGACCGCuUCAu- -3' miRNA: 3'- agUCGGCGuaCG----UCUUGGCGuAGUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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