Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30423 | 3' | -53.4 | NC_006548.1 | + | 35249 | 0.67 | 0.712044 |
Target: 5'- aCCgGUGaAUCc-UCGACuGGCGGCGa -3' miRNA: 3'- cGGgCAC-UAGuuAGCUGuCCGCUGCg -5' |
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30423 | 3' | -53.4 | NC_006548.1 | + | 7999 | 0.66 | 0.723017 |
Target: 5'- cGCgCCG-GAUgAu---GCAGGCGGCGCu -3' miRNA: 3'- -CG-GGCaCUAgUuagcUGUCCGCUGCG- -5' |
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30423 | 3' | -53.4 | NC_006548.1 | + | 35709 | 0.66 | 0.733894 |
Target: 5'- aUCCGUGA-CGAcuaCGACGucGCGACGCu -3' miRNA: 3'- cGGGCACUaGUUa--GCUGUc-CGCUGCG- -5' |
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30423 | 3' | -53.4 | NC_006548.1 | + | 31546 | 0.66 | 0.744663 |
Target: 5'- gGCuCCGUaAUCuAUCaGGCAGGCcaGAUGCu -3' miRNA: 3'- -CG-GGCAcUAGuUAG-CUGUCCG--CUGCG- -5' |
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30423 | 3' | -53.4 | NC_006548.1 | + | 18125 | 0.66 | 0.744663 |
Target: 5'- gGCCUgGUGAUCcAUgcCGACAauccuugguGGCGaACGCa -3' miRNA: 3'- -CGGG-CACUAGuUA--GCUGU---------CCGC-UGCG- -5' |
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30423 | 3' | -53.4 | NC_006548.1 | + | 35092 | 0.66 | 0.744663 |
Target: 5'- aGCUgGUcgaGAUCAa--GGCAGGUGAuCGCa -3' miRNA: 3'- -CGGgCA---CUAGUuagCUGUCCGCU-GCG- -5' |
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30423 | 3' | -53.4 | NC_006548.1 | + | 13265 | 0.66 | 0.765829 |
Target: 5'- cGCUCGguaggCGGUCGAUacauGGGCGAuuCGCu -3' miRNA: 3'- -CGGGCacua-GUUAGCUG----UCCGCU--GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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