Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30430 | 5' | -56.1 | NC_006548.1 | + | 12273 | 1.11 | 0.000473 |
Target: 5'- aGCACCGAACGUAGCGAUCGGCGCCGAc -3' miRNA: 3'- -CGUGGCUUGCAUCGCUAGCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 4764 | 0.77 | 0.136354 |
Target: 5'- gGCGCUucaggugcuuGAGCGUGGCGAUCGGCagGCUGu -3' miRNA: 3'- -CGUGG----------CUUGCAUCGCUAGCCG--CGGCu -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 6688 | 0.75 | 0.16564 |
Target: 5'- gGCGCCGAGaGUGGCGAgaacuucugcaCGGUGCUGAu -3' miRNA: 3'- -CGUGGCUUgCAUCGCUa----------GCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 34749 | 0.75 | 0.174992 |
Target: 5'- uCGCCGAagaucACGUAGUGGcCGGUGCCGu -3' miRNA: 3'- cGUGGCU-----UGCAUCGCUaGCCGCGGCu -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 5132 | 0.75 | 0.189901 |
Target: 5'- uCGCCucgcGGACGUAGCGAaugUCGGgGCUGAc -3' miRNA: 3'- cGUGG----CUUGCAUCGCU---AGCCgCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 33729 | 0.73 | 0.231503 |
Target: 5'- aGUACgCGAugagcuucagugaucGCG-AGCGGUCGGuCGCCGAu -3' miRNA: 3'- -CGUG-GCU---------------UGCaUCGCUAGCC-GCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 28892 | 0.73 | 0.235193 |
Target: 5'- cCACCGGccaaacCGUcgaGGCGGcCGGCGCCGAg -3' miRNA: 3'- cGUGGCUu-----GCA---UCGCUaGCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 21310 | 0.73 | 0.247852 |
Target: 5'- cGCACCGucgGCGgcGgGAauuUCGGUGCUGAu -3' miRNA: 3'- -CGUGGCu--UGCauCgCU---AGCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 28079 | 0.73 | 0.247852 |
Target: 5'- cGCGCCGAAcCGcGGCaGAaacUCGGCcGCCGGa -3' miRNA: 3'- -CGUGGCUU-GCaUCG-CU---AGCCG-CGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 22629 | 0.72 | 0.274853 |
Target: 5'- -uGCCGGaaGCGgaacugcGGCaGAUCGGUGCCGAu -3' miRNA: 3'- cgUGGCU--UGCa------UCG-CUAGCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 20354 | 0.72 | 0.277679 |
Target: 5'- uGCGCCGccuGCGUGacuucuucgucgagcGCGAa-GGCGCCGAa -3' miRNA: 3'- -CGUGGCu--UGCAU---------------CGCUagCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 12609 | 0.72 | 0.289948 |
Target: 5'- aGCGCUcGACGgaaucgaaccaggccAGCuGGUCGGCGCCGGa -3' miRNA: 3'- -CGUGGcUUGCa--------------UCG-CUAGCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 12126 | 0.72 | 0.296613 |
Target: 5'- cGCACCG-ACccAGCaugcUCGGCGCCGGa -3' miRNA: 3'- -CGUGGCuUGcaUCGcu--AGCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 28602 | 0.72 | 0.296613 |
Target: 5'- aGCGCCGcGACcUGGC---CGGCGCCGAg -3' miRNA: 3'- -CGUGGC-UUGcAUCGcuaGCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 9594 | 0.72 | 0.296613 |
Target: 5'- uGCAUCGAGCGcGGCGcgccagguguccAUCGGgGUCGGg -3' miRNA: 3'- -CGUGGCUUGCaUCGC------------UAGCCgCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 2231 | 0.71 | 0.31185 |
Target: 5'- aGCACCuGGuagGCGUAGcCGGUCagcuccGGCGCCGc -3' miRNA: 3'- -CGUGG-CU---UGCAUC-GCUAG------CCGCGGCu -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 5140 | 0.71 | 0.319688 |
Target: 5'- cUugCGGugGUgGGUGAgucCGGCGCCGGu -3' miRNA: 3'- cGugGCUugCA-UCGCUa--GCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 27193 | 0.71 | 0.335806 |
Target: 5'- -uGCCGAGCGUGcucGCGuccagcaggCGGCGaCCGAg -3' miRNA: 3'- cgUGGCUUGCAU---CGCua-------GCCGC-GGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 13391 | 0.7 | 0.361077 |
Target: 5'- aGCGCCGGcggaaucggcgcGCGcuccugcGGCGGgcaaacCGGCGCCGAg -3' miRNA: 3'- -CGUGGCU------------UGCa------UCGCUa-----GCCGCGGCU- -5' |
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30430 | 5' | -56.1 | NC_006548.1 | + | 3683 | 0.7 | 0.378646 |
Target: 5'- aCGCUGAGCGccgaGGCGGUgaacUGGCGCUGGc -3' miRNA: 3'- cGUGGCUUGCa---UCGCUA----GCCGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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