Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30438 | 5' | -58.5 | NC_006548.1 | + | 2870 | 0.66 | 0.452659 |
Target: 5'- -cGCCUgcaGGCGCUCGucgaaggcGAugUUGUCGGCc -3' miRNA: 3'- ucUGGG---UCGCGGGU--------CUugAGCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 21640 | 0.66 | 0.442829 |
Target: 5'- -cGCCCAGgGCuucaccgucCCGGAAaccCUgGUCGGCc -3' miRNA: 3'- ucUGGGUCgCG---------GGUCUU---GAgCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 35744 | 0.66 | 0.43312 |
Target: 5'- -aGCCgCAGCGUCCAGAG-UCcgGUCGaGCg -3' miRNA: 3'- ucUGG-GUCGCGGGUCUUgAG--CAGC-CG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 13138 | 0.66 | 0.432156 |
Target: 5'- uGGACCagGGCGUCCuGAAgggccugggcgcgUUCGUUGGUg -3' miRNA: 3'- -UCUGGg-UCGCGGGuCUU-------------GAGCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 31067 | 0.66 | 0.423536 |
Target: 5'- cGuCCuCGGCGCCCgAGuugagCGUUGGCa -3' miRNA: 3'- uCuGG-GUCGCGGG-UCuuga-GCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 12442 | 0.66 | 0.423536 |
Target: 5'- uGACCagcgcgcgCAGUGCCCAGccuggauugaacGGCUgGUUGGUg -3' miRNA: 3'- uCUGG--------GUCGCGGGUC------------UUGAgCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 6286 | 0.67 | 0.41408 |
Target: 5'- -cGCCCAcggucgaguuGUGCCgGGAGCUUG-CGGUg -3' miRNA: 3'- ucUGGGU----------CGCGGgUCUUGAGCaGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 20726 | 0.67 | 0.41408 |
Target: 5'- uGGCCCAGCaaaaaGCCCAGGAacaUCGcaacgaGGUc -3' miRNA: 3'- uCUGGGUCG-----CGGGUCUUg--AGCag----CCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 13359 | 0.67 | 0.41408 |
Target: 5'- cGGGCaaaCCGGCGCCgAGGGCgguguugaaGUCGcGCa -3' miRNA: 3'- -UCUG---GGUCGCGGgUCUUGag-------CAGC-CG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 21194 | 0.67 | 0.40383 |
Target: 5'- cGGCCUGGcCGCCCAGGcugccgguagcACUgCGcugcugaUCGGCa -3' miRNA: 3'- uCUGGGUC-GCGGGUCU-----------UGA-GC-------AGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 24583 | 0.67 | 0.395564 |
Target: 5'- gAGGCCCAGauCGCCaCAGcGAUguUCuUCGGCa -3' miRNA: 3'- -UCUGGGUC--GCGG-GUC-UUG--AGcAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 26292 | 0.67 | 0.395564 |
Target: 5'- aAGACCUggcgagagcugAGCGCCCAuuuGAACUgG-CGGa -3' miRNA: 3'- -UCUGGG-----------UCGCGGGU---CUUGAgCaGCCg -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 6490 | 0.67 | 0.394653 |
Target: 5'- cGGGCUCGGUcgaaaacGCCCGGAACaUCaUCGaGCg -3' miRNA: 3'- -UCUGGGUCG-------CGGGUCUUG-AGcAGC-CG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 17445 | 0.67 | 0.386509 |
Target: 5'- cGACCCagcagcggcgauGGCGCCgCAGcuggccgaAGCgaugcagccugUCGUCGGCa -3' miRNA: 3'- uCUGGG------------UCGCGG-GUC--------UUG-----------AGCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 26390 | 0.68 | 0.35932 |
Target: 5'- uGGGCUCAaccuguuGCGCCUGGAagaaacACU-GUCGGCg -3' miRNA: 3'- -UCUGGGU-------CGCGGGUCU------UGAgCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 29105 | 0.68 | 0.343329 |
Target: 5'- -cGCCCAGCaGgCCGGu-CUCGUCaGCg -3' miRNA: 3'- ucUGGGUCG-CgGGUCuuGAGCAGcCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 27318 | 0.68 | 0.335121 |
Target: 5'- cGACUCAGCGCUCucucGCUCGcCuGGCc -3' miRNA: 3'- uCUGGGUCGCGGGucu-UGAGCaG-CCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 18574 | 0.68 | 0.319138 |
Target: 5'- uGGACCCGGCGgCCAcgacGGAC-CGggucaUUGGCg -3' miRNA: 3'- -UCUGGGUCGCgGGU----CUUGaGC-----AGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 29363 | 0.69 | 0.311364 |
Target: 5'- cGGAUCgCAGaGCCaaaguGAGCUgGUCGGCg -3' miRNA: 3'- -UCUGG-GUCgCGGgu---CUUGAgCAGCCG- -5' |
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30438 | 5' | -58.5 | NC_006548.1 | + | 30824 | 0.69 | 0.296249 |
Target: 5'- cGAgCCAGCGCCCGGcgggaUCG-CGGa -3' miRNA: 3'- uCUgGGUCGCGGGUCuug--AGCaGCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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