Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30442 | 5' | -50.8 | NC_006548.1 | + | 24524 | 0.67 | 0.793483 |
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Target: 5'- aUCGcuguGGCGAuc-UgGGCCUcGCAGAUCg -3' miRNA: 3'- -AGUu---CCGCUuguAgCCGGA-CGUUUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 28068 | 0.71 | 0.564324 |
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Target: 5'- cUCAcagcAGGCG-GCuggCGGCCUGCAGGc- -3' miRNA: 3'- -AGU----UCCGCuUGua-GCCGGACGUUUag -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 28151 | 0.71 | 0.518514 |
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Target: 5'- --cAGGCGGuugAguUCGGCCUGCAGGc- -3' miRNA: 3'- aguUCCGCU---UguAGCCGGACGUUUag -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 29009 | 0.66 | 0.833564 |
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Target: 5'- cCGuGGCGAGCAgacGCuuCUGCAGGUCg -3' miRNA: 3'- aGUuCCGCUUGUagcCG--GACGUUUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 29482 | 0.67 | 0.76135 |
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Target: 5'- gCGcGGC-AACGUCGGCCaGCG-AUCg -3' miRNA: 3'- aGUuCCGcUUGUAGCCGGaCGUuUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 34067 | 0.77 | 0.250617 |
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Target: 5'- aCGAGGUGAACuggcCGGCCgacagccgggUGCAGAUCa -3' miRNA: 3'- aGUUCCGCUUGua--GCCGG----------ACGUUUAG- -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 34996 | 0.74 | 0.36358 |
|
Target: 5'- -gGAGGCGAGCAaugggCGGCCguaGCAAAg- -3' miRNA: 3'- agUUCCGCUUGUa----GCCGGa--CGUUUag -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 36354 | 0.68 | 0.72785 |
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Target: 5'- gCAu-GCGGcCGUcCGGCCUGCAGAUg -3' miRNA: 3'- aGUucCGCUuGUA-GCCGGACGUUUAg -5' |
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| 30442 | 5' | -50.8 | NC_006548.1 | + | 36530 | 0.77 | 0.257626 |
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Target: 5'- gUCGAGGUGGGC-UCGGCC-GCGauGAUCg -3' miRNA: 3'- -AGUUCCGCUUGuAGCCGGaCGU--UUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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