Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30548 | 5' | -61.1 | NC_006548.1 | + | 35940 | 0.66 | 0.284385 |
Target: 5'- gCCGCCGGcCGcGCGGAuCGcGCCgGCAUu -3' miRNA: 3'- aGGCGGCUaGU-CGUCU-GC-CGGgCGUA- -5' |
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30548 | 5' | -61.1 | NC_006548.1 | + | 21163 | 0.66 | 0.289421 |
Target: 5'- cCCGCCGuaucgugauccauggCAGCucGGACGGCCUGg-- -3' miRNA: 3'- aGGCGGCua-------------GUCG--UCUGCCGGGCgua -5' |
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30548 | 5' | -61.1 | NC_006548.1 | + | 36175 | 0.66 | 0.2916 |
Target: 5'- aCUGCCGG-CAGCcgGGACG-UCCGCAg -3' miRNA: 3'- aGGCGGCUaGUCG--UCUGCcGGGCGUa -5' |
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30548 | 5' | -61.1 | NC_006548.1 | + | 8879 | 0.66 | 0.2916 |
Target: 5'- uUCCGCUGAUCAcacGCuaccuGGCGG-CCGCc- -3' miRNA: 3'- -AGGCGGCUAGU---CGu----CUGCCgGGCGua -5' |
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30548 | 5' | -61.1 | NC_006548.1 | + | 36569 | 0.66 | 0.305702 |
Target: 5'- uUCCGCgucgacgCGAUCAacgccGCGGcCGGCaCCGCGg -3' miRNA: 3'- -AGGCG-------GCUAGU-----CGUCuGCCG-GGCGUa -5' |
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30548 | 5' | -61.1 | NC_006548.1 | + | 29186 | 0.66 | 0.305702 |
Target: 5'- gCUGCCGcgCGGCcaguucgcugacgAGAcCGGCCUGCu- -3' miRNA: 3'- aGGCGGCuaGUCG-------------UCU-GCCGGGCGua -5' |
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30548 | 5' | -61.1 | NC_006548.1 | + | 18796 | 0.66 | 0.32189 |
Target: 5'- -gCGUCGAgCAGC--ACGGCCUGCGg -3' miRNA: 3'- agGCGGCUaGUCGucUGCCGGGCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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