miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30548 5' -61.1 NC_006548.1 + 12227 0.68 0.231689
Target:  5'- gCCGCuCGAaccagcacgCGGCGauacugcucGGCGGCCCGCGg -3'
miRNA:   3'- aGGCG-GCUa--------GUCGU---------CUGCCGGGCGUa -5'
30548 5' -61.1 NC_006548.1 + 4183 0.69 0.182492
Target:  5'- cUCCGCgGcauUCGGCAGACcaCCCGCGg -3'
miRNA:   3'- -AGGCGgCu--AGUCGUCUGccGGGCGUa -5'
30548 5' -61.1 NC_006548.1 + 27068 0.69 0.182492
Target:  5'- gUCUGCUGAUCGGCGGACucGCCgaCGCu- -3'
miRNA:   3'- -AGGCGGCUAGUCGUCUGc-CGG--GCGua -5'
30548 5' -61.1 NC_006548.1 + 23595 0.7 0.168266
Target:  5'- aCCGCCuggcggGGUCAGCGGGucaucCGGCCCGa-- -3'
miRNA:   3'- aGGCGG------CUAGUCGUCU-----GCCGGGCgua -5'
30548 5' -61.1 NC_006548.1 + 20853 0.7 0.150847
Target:  5'- uUCUGgCGcggCAGCAGGCGGCCgGCc- -3'
miRNA:   3'- -AGGCgGCua-GUCGUCUGCCGGgCGua -5'
30548 5' -61.1 NC_006548.1 + 1053 0.73 0.088588
Target:  5'- aUCGCUcGUCAGCAGcuCGGCCUGCAg -3'
miRNA:   3'- aGGCGGcUAGUCGUCu-GCCGGGCGUa -5'
30548 5' -61.1 NC_006548.1 + 18796 0.66 0.32189
Target:  5'- -gCGUCGAgCAGC--ACGGCCUGCGg -3'
miRNA:   3'- agGCGGCUaGUCGucUGCCGGGCGUa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.