Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30548 | 5' | -61.1 | NC_006548.1 | + | 35985 | 0.67 | 0.24404 |
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Target: 5'- cUCGCCGGUCGGUA---GGCCCGgAUa -3' miRNA: 3'- aGGCGGCUAGUCGUcugCCGGGCgUA- -5' |
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| 30548 | 5' | -61.1 | NC_006548.1 | + | 12227 | 0.68 | 0.231689 |
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Target: 5'- gCCGCuCGAaccagcacgCGGCGauacugcucGGCGGCCCGCGg -3' miRNA: 3'- aGGCG-GCUa--------GUCGU---------CUGCCGGGCGUa -5' |
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| 30548 | 5' | -61.1 | NC_006548.1 | + | 4183 | 0.69 | 0.182492 |
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Target: 5'- cUCCGCgGcauUCGGCAGACcaCCCGCGg -3' miRNA: 3'- -AGGCGgCu--AGUCGUCUGccGGGCGUa -5' |
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| 30548 | 5' | -61.1 | NC_006548.1 | + | 27068 | 0.69 | 0.182492 |
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Target: 5'- gUCUGCUGAUCGGCGGACucGCCgaCGCu- -3' miRNA: 3'- -AGGCGGCUAGUCGUCUGc-CGG--GCGua -5' |
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| 30548 | 5' | -61.1 | NC_006548.1 | + | 23595 | 0.7 | 0.168266 |
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Target: 5'- aCCGCCuggcggGGUCAGCGGGucaucCGGCCCGa-- -3' miRNA: 3'- aGGCGG------CUAGUCGUCU-----GCCGGGCgua -5' |
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| 30548 | 5' | -61.1 | NC_006548.1 | + | 20853 | 0.7 | 0.150847 |
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Target: 5'- uUCUGgCGcggCAGCAGGCGGCCgGCc- -3' miRNA: 3'- -AGGCgGCua-GUCGUCUGCCGGgCGua -5' |
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| 30548 | 5' | -61.1 | NC_006548.1 | + | 1053 | 0.73 | 0.088588 |
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Target: 5'- aUCGCUcGUCAGCAGcuCGGCCUGCAg -3' miRNA: 3'- aGGCGGcUAGUCGUCu-GCCGGGCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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