miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30548 5' -61.1 NC_006548.1 + 29186 0.66 0.305702
Target:  5'- gCUGCCGcgCGGCcaguucgcugacgAGAcCGGCCUGCu- -3'
miRNA:   3'- aGGCGGCuaGUCG-------------UCU-GCCGGGCGua -5'
30548 5' -61.1 NC_006548.1 + 29436 0.68 0.237797
Target:  5'- gUUGCCGcgCAGCAGcUGGCCCagaagGCAa -3'
miRNA:   3'- aGGCGGCuaGUCGUCuGCCGGG-----CGUa -5'
30548 5' -61.1 NC_006548.1 + 33298 0.67 0.250418
Target:  5'- cCCGCCuGGUCGGCAaauugcGGCaGCCCGguUu -3'
miRNA:   3'- aGGCGG-CUAGUCGU------CUGcCGGGCguA- -5'
30548 5' -61.1 NC_006548.1 + 35940 0.66 0.284385
Target:  5'- gCCGCCGGcCGcGCGGAuCGcGCCgGCAUu -3'
miRNA:   3'- aGGCGGCUaGU-CGUCU-GC-CGGgCGUA- -5'
30548 5' -61.1 NC_006548.1 + 35985 0.67 0.24404
Target:  5'- cUCGCCGGUCGGUA---GGCCCGgAUa -3'
miRNA:   3'- aGGCGGCUAGUCGUcugCCGGGCgUA- -5'
30548 5' -61.1 NC_006548.1 + 36175 0.66 0.2916
Target:  5'- aCUGCCGG-CAGCcgGGACG-UCCGCAg -3'
miRNA:   3'- aGGCGGCUaGUCG--UCUGCcGGGCGUa -5'
30548 5' -61.1 NC_006548.1 + 36569 0.66 0.305702
Target:  5'- uUCCGCgucgacgCGAUCAacgccGCGGcCGGCaCCGCGg -3'
miRNA:   3'- -AGGCG-------GCUAGU-----CGUCuGCCG-GGCGUa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.