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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30553 | 5' | -57.7 | NC_006548.1 | + | 29105 | 0.68 | 0.361992 |
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Target: 5'- cGCCcaGCAGGCCGGuCUCGucagcgaacugGCCGCg-- -3' miRNA: 3'- -CGGa-CGUCCGGCUuGAGU-----------UGGCGgac -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 29155 | 0.66 | 0.456154 |
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Target: 5'- gGCCUGCugggcGGCCuGGauACUCGACU-CCUGc -3' miRNA: 3'- -CGGACGu----CCGG-CU--UGAGUUGGcGGAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 29404 | 0.66 | 0.466284 |
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Target: 5'- uCCUGCAGGCgGucAGC-CGAUCGCUg- -3' miRNA: 3'- cGGACGUCCGgC--UUGaGUUGGCGGac -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 29452 | 0.67 | 0.388796 |
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Target: 5'- cGCCUGCAGGaaCGAuACcgCGGCCuCCUGc -3' miRNA: 3'- -CGGACGUCCg-GCU-UGa-GUUGGcGGAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 29562 | 0.67 | 0.425527 |
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Target: 5'- aCC-GCAGGCgGAACUCugguggcuggugcGGCUGCCa- -3' miRNA: 3'- cGGaCGUCCGgCUUGAG-------------UUGGCGGac -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 32796 | 0.66 | 0.446143 |
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Target: 5'- aGCCUGCaAGGCUGAGgUCGaggaGCaCGCgCUc -3' miRNA: 3'- -CGGACG-UCCGGCUUgAGU----UG-GCG-GAc -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 32899 | 0.71 | 0.217025 |
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Target: 5'- cGCCaGCGGGUauCGcACUCGACCGUUUGu -3' miRNA: 3'- -CGGaCGUCCG--GCuUGAGUUGGCGGAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 36368 | 0.72 | 0.189538 |
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Target: 5'- gGCCUGCAGaugaacuacgcGCU--GCUCAGCCGCgUGg -3' miRNA: 3'- -CGGACGUC-----------CGGcuUGAGUUGGCGgAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 36416 | 0.74 | 0.143633 |
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Target: 5'- aUCUGCAGGCCGGACg--GCCGCa-- -3' miRNA: 3'- cGGACGUCCGGCUUGaguUGGCGgac -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 36916 | 0.66 | 0.436256 |
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Target: 5'- -gCUGCAGGCCGucAACUgguUGACuCGUCUGc -3' miRNA: 3'- cgGACGUCCGGC--UUGA---GUUG-GCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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